Ada2b, but not Ada2a, copurifies with Taf4, Gcn5 and e(y)1 in a large complex, approximately 2 MDa in size.

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Embryonic nuclear extract was first fractionated on a heparin column, then size fractioned by Superose 6 gel filtration.