Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was purified embryonic centrosomes of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Centrosomes were isolated from highly mitotic syncytial embryos and subjected to cnn protein affinity purification.
Phosphopeptides in the affinity purified fraction were enriched by titanium dioxide affinity chromatography.
Two-hybrid system: yeast GAL4-BD/VP16-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/VP16-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
