Invitrogen
New stable cell line derived from this cell line: The following derivative cell lines were generated: Parental OsTir-expressing S2 cell line, Trf2 C-terminally-tagged AID S2 cell line, Tbp-N-terminally-tagged AID S2 cell line, Dref-N-terminally-tagged AID S2 cell line, TfIIA-L-C-terminally-tagged AID S2 cell line, Chro-N-terminally-tagged AID S2 cell line.
New stable cell line derived from this cell line: Stable S2 cell lines were created to study chromatin remodeling. The base S2[OsTir1] was created and selected core proteins from the four main sub-families of ATP-dependent chromatin remodelers (Snr1, Iswi, Ino80, and Chd1) as well as SWI/SNF component Brd7-9 were inserted to create S2OsTir1-Snr1-AID, S2OsTir1-Iswi-AID, S2OsTir1-Ino80-AID, S2OsTir1-Chd1-AID, and S2OsTir1-Brd7-AID for component depletion studies.
New stable cell line derived from this cell line: Centriolar proteins were tagged with GFP at either the N- or C- terminus and used to create stable S2 cell lines. S2 cell lines constitutively expressing the following constructs were created: asl-GFP, Sas-6-GFP, ana1-GFP, GFP-ana1, Cep135-GFP, ana3-GFP, Rcd4-GFP, ana2-GFP, GFP-asl.
New stable cell line derived from this cell line: A stable S2- mCherry:Tubulin-GFP:cid cell line was created. This was accomplished by transfecting cells stably expressing GFP:CID (from Gary Karpen) with inducible mCherry-alpha-tubulin.
New stable cell line derived from this cell line: A stable S2 cell line expressing GFP-Lam and mCherry-alpha-tubulin was created. Stable S2 lines expressing RFP(Unk)-Lam wild type and constructs in which the CDK sites were mutated were generated. A stable S2 cell line that expressed GFP-baf and mCherry-alpha-tubulin was created.
New stable cell line derived from this cell line: Stable cell lines were constructed for expression of brm constructs (recBRM-V5 and recBRM-K804R-V5).
S2 cells obtained from Invitrogen. Many features of the S2 cell line suggest that it is derived from a macrophage-like lineage.
New stable cell line derived from this cell line: Stable S2 cell lines expressing Tag:polyHis-tagged-PGRP-LF were generated.
New stable cell line derived from this cell line: Stable S2 cell lines overexpressing an N-terminal fragment of hh were generated.
New stable cell line derived from this cell line: Stable S2 cell lines expressing Human ADAM33 under the control of the Act5C or metallothionein promoter were generated.