FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Su(var)3-7, Su(var)205
FlyBase ID
FBig0000097100
Interaction Network
Interactions Browser links
Su(var)3-7 network
Su(var)205 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0098579-1.coIP.WB
Reference
Cleard et al., 1997
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Su(var)3-7 

SU(VAR)3-7

bait
Su(var)205 

HP1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Cleard et al., 1997)
FBrf0131282-1.Y2H
Reference
Delattre et al., 2000
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 17blastoderm stagegastrula stageembryonic stage 8dorsal closure stageembryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic cycle 9embryonic stage 2late extended germ band stageembryonic stage 5embryonic stage 11embryonic stage 3embryonic stage 12embryonic stage 15embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7embryonic stage 16
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Su(var)3-7 

Su(var)3-7

bait

fused to LexA DNA-binding domain

Su(var)3-7 
Su(var)205 

HP1

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Su(var)3-7 
zinc fingers
sufficient binding region
aa 189-844
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Delattre et al., 2000)

A cDNA encoding Su(var)205 was one of 50 Su(var)3-7-interacting clones.

(Delattre et al., 2000)

Two-hybrid system: yeast LexA-BD/B42-AD

(Delattre et al., 2000)
FBrf0131282-3.Y2H
Reference
Delattre et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Su(var)3-7 

Su(var)3-7

bait

fused to LexA DNA-binding domain

Su(var)3-7 
Su(var)205 

HP1

prey

fused to B42 activation domain

Su(var)205 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Su(var)3-7 
region
sufficient binding region
aa 266-334aa 573-612aa 845-971
Su(var)205 
C-terminal region
sufficient binding region
aa 95-206
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Delattre et al., 2000)

Multiple regions of Su(var)3-7 bind Su(var)205.

(Delattre et al., 2000)

Two-hybrid system: yeast LexA-BD/B42-AD

(Delattre et al., 2000)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)