Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
All immunoprecipitations were performed in the presence of ethidium bromide in order to disrupt protein DNA interactions.
Bait derived from a transgenic line carrying a FLAG-tagged lin-52 construct in a lin-52[93] homozygous mutant background.
Prey endogenous to embryonic nuclear extract and coimmunoprecipitated with FLAG-tagged lin-52.
lin-52 fractionates exclusively with components of the MMB/dREAM complex.
Proteins associated with affinity purified FLAG-tagged lin-52 were subjected to gel filtration.
Bait derived from a transgenic line carrying a FLAG-tagged lin-52 construct in a lin-52[93] homozygous mutant background.
Prey endogenous to embryonic nuclear extract.
