Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

All immunoprecipitations were performed in the presence of ethidium bromide in order to disrupt protein DNA interactions.

Bait derived from a transgenic line carrying a FLAG-tagged lin-52 construct in a lin-52[93] homozygous mutant background.

Prey endogenous to embryonic nuclear extract and coimmunoprecipitated with FLAG-tagged lin-52.

Myb associates with lin-52 as part of the MMB/dREAM complex.

lin-52 fractionates exclusively with components of the MMB/dREAM complex.

Proteins associated with affinity purified FLAG-tagged lin-52 were subjected to gel filtration.

Bait derived from a transgenic line carrying a FLAG-tagged lin-52 construct in a lin-52[93] homozygous mutant background.

Prey endogenous to embryonic nuclear extract.

Binding of Myb to a mip130-lin-52 heterodimer was captured as two binary interactions.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.