Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

Interspecific interaction of Dmel\exd with Mmus\Meis1 (MEIS1), orthologous to Dmel\hth (inferred by curator).

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

Interspecific interaction of Dmel\exd with Mmus\Meis1 (MEIS1), orthologous to Dmel\hth (inferred by curator).

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

hth stimulates weak binding of lab DNA by exd. Alone, neither hth nor exd binds to lab DNA.

Various combinations of hth, lab and exd proteins were mixed with [32]P labeled lab DNA. DNA-protein complexes observed by EMSA were used to deduce protein-protein interactions.

Bait endogenous to embryonic extract.

Prey endogenous to embryonic extract.

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

exd and hth homeodomainless and full length proeins were mixed with [32]P labeled DNA. The homeodomainless hth isoform is capable of interacting with exd, but at a reduced level compared to full length.

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

Embryos were collected overnight and incubated at 4 degrees C for 28 hrs prior to BiFC detection.

Interaction in vitro; proteins produced by in vitro transcription/translation.

exd and hth were mixed with [32]P labeled DNA. DNA-protein complexes observed by EMSA were used to deduce protein-protein interactions.

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

exd and hth form heterodimers that bind DNA. Neither binds DNA alone.

Various combinations of hth, lab and exd proteins were mixed with [32]P labeled DNA. DNA-protein complexes observed by EMSA were used to deduce protein-protein interactions.

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system;

Electrophoretic mobility shift assays (EMSA) revealed that exd/hth could bind specifically to an evolutionarily conserved 52 base pair sequence within the Act88F enhancer region, and that DNA binding required functional homeodomains for both exd and hth, indicating that these factors can form a complex on the Act88F enhancer sequence.

Source was eye disc of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

Positive control.

Source was cell extract of S2R+ cell line; proteins produced from transfected construct or endogenous gene.

HGScore = 26.630672

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD