brm and Snr1 are also present in large molecular mass complexes, as determined by gel filtration chromatography of embryonic nuclear extracts.

This interaction persists in the presence of ethidium bromide, and so is not due to indirect interactions via DNA.

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

brm, mor, dalao, Hsc70-4, Bap60, Bap55, Snr1 and Bap47 (actin) were identified as components of the Brahma complex.

Nuclear extract was fractionated through several different chromatographic steps, selecting for brm-containing fractions, before pull down of His tagged brm.

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

Nuclear extract was fractionated on a glycerol gradient and fractions containing brm and osa were used for immunoprecipitation.

brm coimmunoprecipitates osa and Snr1 (Brahma complex), but not Iswi.

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

brm, osa, Bap170, p400, mor, dalao, Bap60, Bap55, Snr1, Bap47 (actin) and Bap26 were identified as components of the Brahma complex.

Source was embryonic nuclear extract of wild-type fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

Nuclear extract was fractionated through several different chromatographic steps, selecting for brm-containing fractions, before immunoprecipitation of brm.

The E1 mutant form of Snr1 carrying a G256D substitution is capable of interacting with brm.

Source was pupal extracts; bait produced from endogenous gene; prey produced from endogenous gene

brm, mor, dalao, Bap60, Bap55, Snr1 and actin form the Brahma (BRM) complex, together with either osa (BAP BRM complex) or polybromo and Bap170 (PBAP BRM complex).

Source was embryonic nuclear extract wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Nuclear extract was fractionated by ammonium sulfate precipitation and several chromatographic steps before being subjected to immunoprecipitation.

Embryonic extracts were prepared from flies expressing MYC-tagged DmCDK2

Source was embryonic extracts; bait produced endogenous gene; prey derived from transgenic construct

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from endogenous gene.

Embryonic extracts were prepared from flies expressing MYC-tagged DmCDK2