Source was embryonic extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Identical results were obtained with ovary nuclear extract. IPs were either performed with or without ethidium bromide in the extracts before precipitation to demonstrate that DNA was unnecessary for interaction.
Source was embryonic extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Identical results were obtained with ovary nuclear extract. IPs were either performed with or without ethidium bromide in the extracts before precipitation to demonstrate that DNA was unnecessary for interaction.
Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
All immunoprecipitations were performed in the presence of ethidium bromide in order to disrupt protein DNA interactions.
Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
All immunoprecipitations were performed in the presence of ethidium bromide in order to disrupt protein DNA interactions.
Source was testis of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
