Pk91C, 1J
kinase that coordinates with Polo for mitotic progression - absence of Gwl during meiosis results in excessive and/or premature Polo activity leading to premature loss of sister chromatid cohesion
Please see the JBrowse view of Dmel\gwl for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.44
None of the polypeptides share 100% sequence identity.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\gwl using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: maternally deposited
JBrowse - Visual display of RNA-Seq signals
View Dmel\gwl in JBrowse3-64
3-63.4
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
monoclonal
gwl is required for sister chromatid cohesion in female meiosis I.
gwl has multiple roles in progression through mitosis and meiosis.
When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of G2/M phase cells, an increase in mitotic index, a decrease in the ratio of cells in prometaphase and metaphase versus the total number of mitotic cells, a whole range of mitotic abnormalities, spindle abnormalities, chromosome abnormalities, chromosome segregation defects are seen.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Mutants exhibit a high mitotic index and metaphase arrest.
Isolated from a genomic library using a Pka-C1 probe.
gwl may encode a protein kinase.
Gene in D.melanogaster encoding product related (by sequence comparison) to the serine-threonine protein kinases of mammals. Isolated from Drosophila clones obtained with mammalian probes.
Source for merge of: Pk91C CG7719
Source for merge of: gwl scant
Source for identity of: gwl Pk91C
The gene is named "greatwall" because the mutant phenotype suggests a role in protecting chromosome structure.
