CK2, CKII, CK2β, And, Andante
CkII catalytic and regulatory subunits - modulates Antennapedia's properties, restricting its activity to an appropriate level - a regulator of the active zone protein Bruchpilot - stabilizes Clock and represses its activity in circadian oscillator - promotes Hedgehog signaling by regulating both smoothened and Cubitus interrupt Myc and Casein kinase 2 target mushroom body miniature, which is required for ribosome biogenesis and cell growth of central brain neuroblasts
Please see the JBrowse view of Dmel\CkIIβ for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.46
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
0.946 (longest cDNA)
215 (aa); 24.7 (kD predicted)
28.2 (kD observed)
Tetramer of two alpha and two beta subunits.
Phosphorylated by alpha subunit.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CkIIβ using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
JBrowse - Visual display of RNA-Seq signals
View Dmel\CkIIβ in JBrowse1-36
1-36.8
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
Splice isoforms CkIIβ-VIIc and -VIId are dispensable for viability and for formation of normal mushroom bodies.
CkIIβ dependant phosphorylation has a role in the circadian rhythm.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
The number of neurons forming the adult mushroom body is greatly reduced in CkIIβ hypomorphs.
Mutations in CkIIβ reduce the number of mushroom body neurons (Kenyon cells).
Casein kinase II specifically phosphorylates a set of serine residues within the cact PEST domain.
Casein kinase II phosphorylates Ser468 (a residue in the PEST domain) of cact in vitro.
Genetic mosaic analysis identifies the tissues that require the CkIIβ product.
Serine 2 and Serine 4 residues are major sites of autophosphorylation of the β subunit of casein kinase (as shown by the construction of A2 and A4 mutants in vitro). Autophosphorylation of this subunit reduces the phosphorylation of some substrate proteins in vitro (calmodulin, elongation factor 1, glycogen synthase) but not of others (casein).
Recombinant CkII in vitro binds to spermine and this interaction is concomitant with a striking effect on the structural polymeric organisation on the kinase which in the presence of the polyamine exhibits a ring-like structure.
Chimeric β subunits of human and Drosophila cDNA reveal that the N terminal region of the CkIIβ subunit is responsible for the requirement for the redox state during renaturation, and is also responsible for solubility and electrophoretic mobility. of S.cerevisiae deleted for both genes.
Recombinant Casein kinase reconstituted from human and Drosophila, α and β subunits demonstrate that CkIIβ is responsible for renaturation and reconstitution of Casein kinase II dependent on the redox conditions.
Casein kinase II-mediated phosphorylation of Top2 stimulates its activity by enhancing the ability of the enzyme to hydrolyse its high energy ATP cofactor.
Source for merge of: CkIIβ mbu
The 'Andante' mutant chromosome carries two independent EMS-induced mutations, one in dy (dyQ189stop) and one in CkIIβ (CkIIβAnd). The dyQ189stop mutation has no effect on rhythmicity, rather the CkIIβAnd allele is responsible for the circadian long-period phenotype of the mutant chromosome.
Drosophila Casein kinase II α and β subunits can rescue the lethality of S.cerevisiae deleted for both genes.
