UASt regulatory sequences drive expression of the Nuak coding sequence, mutated to carry a phospho-mimetic T226E amino acid substitution within the T-loop region of the kinase domain.
partially lethal - majority live, with NuakK99R, Scer\GAL4Mef2.PR
NuakT226E.UAS/Scer\GAL4Mef2.PR partially rescues NuakK99R