UASp regulatory sequences drive expression of a fusion protein in which the N-terminal 46 amino acids of Incenp (the Borealin-Survivin domain) have been replaced with borr coding sequence that lacks the C-terminal domain (only borr amino acids 1-17 are present). In addition, eight silent mutations have been introduced into the region corresponding to Incenp amino acids 437-441; this renders the construct resistant to RNAi mediated by P{TRiP.GL00279}.
IncenpGL00279, Scer\GAL4VP16.mat.αTub67C, borr::IncenpΔC.UASp.RR has female sterile phenotype
IncenpGL00279, Scer\GAL4VP16.mat.αTub67C, borr::IncenpΔC.UASp.RR has oocyte phenotype
IncenpGL00279, Scer\GAL4VP16.mat.αTub67C, borr::IncenpΔC.UASp.RR has meiotic spindle | oogenesis phenotype
IncenpGL00279, Scer\GAL4VP16.mat.αTub67C, borr::IncenpΔC.UASp.RR has meiotic spindle midzone | oogenesis | absent phenotype