FlyBase Allele Report: Dmel\Gcna[KO]

General Information

Symbol

Dmel\Gcna^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0363739

Feature type

allele

Associated gene

Dmel\Gcna

Associated Insertion(s)

TI{TI}Gcna^KO

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Bhargava et al., 2020)

Mutagen

CRISPR/Cas9

(Bhargava et al., 2020)

gene targeting by homologous recombination

(Bhargava et al., 2020)

Progenitor genotype

Associated Insertion(s)

TI{TI}Gcna^KO

Cytology

Description

Almost the entire Gcna coding sequence has been deleted. The deleted sequence has been replaced by a cassette that contains a Disc\RFP^{DsRed(T1).3xP3.cHa} marker flanked by loxP sites (allowing subsequent removal of this marker from a targeted locus) and a single attP site.

(Bhargava et al., 2020)

Allele components

Component

Use(s)

Also carries

attP

integrase target site

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | female | progressive (with Df(1)BSC719)

(Bhargava et al., 2020)

partially lethal | maternal effect

(Bhargava et al., 2020)

some die during embryonic stage | maternal effect

(Bhargava et al., 2020)

visible | adult stage | maternal effect

(Bhargava et al., 2020)

Phenotype Manifest In

egg chamber (with Df(1)BSC719)

(Bhargava et al., 2020)

embryo | maternal effect

(Bhargava et al., 2020)

germarium (with Df(1)BSC719)

(Bhargava et al., 2020)

nucleus | embryonic stage | maternal effect

(Bhargava et al., 2020)

oocyte (with Df(1)BSC719)

(Bhargava et al., 2020)

ovariole

(Bhargava et al., 2020)

ovary

(Bhargava et al., 2020)

ovary (with Df(1)BSC719)

(Bhargava et al., 2020)

ring canal (with Df(1)BSC719)

(Bhargava et al., 2020)

Detailed Description

Statement

Reference

Gcna^KO homozygous females initially lay eggs but stop after approximately one week.

Ovarioles of Gcna^KO exhibit more abundant γH2Av foci (DNA damage marker) per nuclei.

Ovaries of Gcna^KO and embryos derived from those mothers exhibit altered karyotypes.

Ovaries of Gcna^KO/Df(1)BSC719 exhibit egg chambers deviated from the normally invariant number of 16 germ cells per cyst (which becomes more penetrant with age), defects in differentiation, delayed oocyte specification, germarium with larger and more abundant γH2Av foci per nuclei, and early egg chambers with γH2Av staining and aberrant accumulation of p53 (which correlates with DNA damage).

Embryos derived from Gcna^KO mothers exhibit numerous mitotic defects during early embryogenesis including chromosome tangling, micronucleus formation, nuclear fusion, chromosome segregation defects and disruption of cell-cycle synchrony.

Adults derived from Gcna^KO mothers appear sickly and sub-fertile. Some of them display bilateral gynandromorphism.

(Bhargava et al., 2020)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Gcna^KO has increased mortality during development phenotype, enhanceable by mh¹

(Bhargava et al., 2020)

NOT Enhancer of

Statement

Reference

Gcna^KO is a non-enhancer of lethal - all die during embryonic stage | maternal effect phenotype of mh¹

(Bhargava et al., 2020)

NOT Suppressor of

Statement

Reference

Gcna^KO is a non-suppressor of lethal - all die during embryonic stage | maternal effect phenotype of mh¹

(Bhargava et al., 2020)

Phenotype Manifest In

Enhanced by

Statement

Reference

Gcna^KO has ovariole phenotype, enhanceable by mh¹

(Bhargava et al., 2020)

Suppressed by

Statement

Reference

Gcna^KO has ovariole phenotype, suppressible by mei-W68⁰⁹⁴⁹

(Bhargava et al., 2020)

Gcna^KO has ovariole phenotype, suppressible by mei-P22^P22

(Bhargava et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Gcna^KO is partially rescued by Gcna^{HE-AA.UASp.Tag:HA}/Scer\GAL4^nanos.PI

(Bhargava et al., 2020)

Gcna^KO is partially rescued by Scer\GAL4^nanos.PI/Gcna^UASp.Tag:HA

(Bhargava et al., 2020)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Gcna^KO

(Bhargava et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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