Almost the entire Gcna coding sequence has been deleted. The deleted sequence has been replaced by a cassette that contains a Disc\RFPDsRed(T1).3xP3.cHa marker flanked by loxP sites (allowing subsequent removal of this marker from a targeted locus) and a single attP site.
oocyte (with Df(1)BSC719)
ovary (with Df(1)BSC719)
GcnaKO homozygous females initially lay eggs but stop after approximately one week.
Ovarioles of GcnaKO exhibit more abundant γH2Av foci (DNA damage marker) per nuclei.
Ovaries of GcnaKO and embryos derived from those mothers exhibit altered karyotypes.
Ovaries of GcnaKO/Df(1)BSC719 exhibit egg chambers deviated from the normally invariant number of 16 germ cells per cyst (which becomes more penetrant with age), defects in differentiation, delayed oocyte specification, germarium with larger and more abundant γH2Av foci per nuclei, and early egg chambers with γH2Av staining and aberrant accumulation of p53 (which correlates with DNA damage).
Embryos derived from GcnaKO mothers exhibit numerous mitotic defects during early embryogenesis including chromosome tangling, micronucleus formation, nuclear fusion, chromosome segregation defects and disruption of cell-cycle synchrony.
Adults derived from GcnaKO mothers appear sickly and sub-fertile. Some of them display bilateral gynandromorphism.
GcnaKO has increased mortality during development phenotype, enhanceable by mh1
GcnaKO is a non-enhancer of lethal - all die during embryonic stage | maternal effect phenotype of mh1
GcnaKO is a non-suppressor of lethal - all die during embryonic stage | maternal effect phenotype of mh1
GcnaKO has ovariole phenotype, suppressible by mei-W680949
GcnaKO has ovariole phenotype, suppressible by mei-P22P22
GcnaKO is partially rescued by GcnaHE-AA.UASp.Tag:HA/Scer\GAL4nanos.PI
GcnaKO is partially rescued by Scer\GAL4nanos.PI/GcnaUASp.Tag:HA