FlyBase Allele Report: Dmel\raw[dcp-1]

General Information

Symbol

Dmel\raw^dcp-1

Species

D. melanogaster

Name

FlyBase ID

FBal0358021

Feature type

allele

Associated gene

Dmel\raw

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Hao et al., 2019)

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: A532D.

(Hao et al., 2019)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:8,712,009..8,712,009 [-]

Nucleotide change:

C8712009A

Amino acid change:

Reported amino acid change:

A532D

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(Hao et al., 2019)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

axon | third instar larval stage | conditional

(Hao et al., 2019)

axon | third instar larval stage | conditional (with Df(2L)BSC204)

(Hao et al., 2019)

axon | third instar larval stage | conditional (with raw^134.47)

(Hao et al., 2019)

axon | third instar larval stage | conditional (with raw^155.27)

(Hao et al., 2019)

embryonic/larval motor neuron | third instar larval stage | conditional

(Hao et al., 2019)

embryonic/larval motor neuron | third instar larval stage | conditional (with Df(2L)BSC204)

(Hao et al., 2019)

embryonic/larval motor neuron | third instar larval stage | conditional (with raw^134.47)

(Hao et al., 2019)

embryonic/larval motor neuron | third instar larval stage | conditional (with raw^155.27)

(Hao et al., 2019)

embryonic/larval neuromuscular junction | third instar larval stage | conditional

(Hao et al., 2019)

embryonic/larval neuromuscular junction | third instar larval stage | conditional (with raw^134.47), with Scer\GAL4^BG380, raw^UASp.RA

(Hao et al., 2019)

embryonic/larval neuromuscular junction | third instar larval stage | conditional (with raw^134.47), with Scer\GAL4^Toll-6-D42, raw^UASp.RA

(Hao et al., 2019)

larval multidendritic class IV neuron | third instar larval stage | conditional

(Hao et al., 2019)

Detailed Description

Statement

Reference

raw^dcp-1 in transheterozygosity with Df(2L)BSC204, but not with Df(2L)BSC688, Df(2L)Exel6011, Df(2L)BSC109, Df(2L)BSC354 or Df(2L)ED775, provides protection against (injury-induced) Wallerian degeneration of third instar larval axons.

raw^dcp-1 homozygotes show protection against (injury-induced) Wallerian degeneration of third instar larval motor and class IV sensory neurons; axons and synapses remain intact for at least 48h after injury and axonal growth at 20h after injury is similar to uninjured controls; neuromuscular junctions exhibit robust evoked EJPs, spontaneous mEJPs and quantal content at 20h after injury, similar to uninjured controls.

raw^dcp-1/raw^134.47 and raw^dcp-1/raw^155.27 transheterozygotes also show protection against (injury-induced) Wallerian degeneration of third instar larval motor neurons.

Unlike controls, raw^dcp-1/raw^134.47 transheterozygotes expressing raw^UASp.RA under the control of Scer\GAL4^Toll-6-D42 or Scer\GAL4^BG380 sustain some neurotransmission at the third instar larval neuromuscular junction at 20h after injury.

(Hao et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

raw^dcp-1 has abnormal neuroanatomy | recessive | third instar larval stage | conditional phenotype, suppressible | partially by hiw^ΔN

(Hao et al., 2019)

raw^dcp-1 has abnormal neuroanatomy | recessive | third instar larval stage | conditional phenotype, suppressible | partially by Scer\GAL4^tey-5053A/kay^Fbz.UAS

(Hao et al., 2019)

raw^dcp-1 has abnormal neuroanatomy | recessive | third instar larval stage | conditional phenotype, suppressible | partially by Scer\GAL4^tey-5053A/Jra^Jbz.UAS

(Hao et al., 2019)

NOT suppressed by

Statement

Reference

raw^dcp-1 has abnormal neuroanatomy | recessive | third instar larval stage | conditional phenotype, non-suppressible by Scer\GAL4^tey-5053A/Nmnat^GD8082

(Hao et al., 2019)

raw^dcp-1 has abnormal neuroanatomy | recessive | third instar larval stage | conditional phenotype, non-suppressible by Scer\GAL4^tey-5053A/bsk^K53R.UAS

(Hao et al., 2019)

NOT Enhancer of

Statement

Reference

raw^dcp-1/raw^134.47 is a non-enhancer of abnormal neuroanatomy | larval stage phenotype of Hsap\SARM1^{SAM.TIR.UAS.Venus}, Scer\GAL4^tey-5053A

(Waller and Collins, 2023)

NOT Suppressor of

Statement

Reference

raw^dcp-1/raw^134.47 is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of Hsap\SARM1^{SAM.TIR.UAS.Venus}, Scer\GAL4^tey-5053A

(Waller and Collins, 2023)

Other

Statement

Reference

Scer\GAL4^tey-5053A, raw^dcp-1, wnd^GL00282 has abnormal neuroanatomy | third instar larval stage phenotype

(Hao et al., 2019)

Scer\GAL4^tey-5053A, kay^Fbz.UAS, raw^dcp-1 has abnormal neuroanatomy | third instar larval stage phenotype

(Hao et al., 2019)

Scer\GAL4^tey-5053A, bsk^K53R.UAS, raw^dcp-1 has abnormal neuroanatomy | third instar larval stage phenotype

(Hao et al., 2019)

Jra^Jbz.UAS, Scer\GAL4^tey-5053A, raw^dcp-1 has abnormal neuroanatomy | third instar larval stage phenotype

(Hao et al., 2019)

Phenotype Manifest In

Suppressed by

Statement

Reference

raw^dcp-1 has embryonic/larval motor neuron | third instar larval stage | conditional phenotype, suppressible | partially by hiw^ΔN

(Hao et al., 2019)

raw^dcp-1 has axon | third instar larval stage | conditional phenotype, suppressible | partially by Scer\GAL4^tey-5053A/Jra^Jbz.UAS

(Hao et al., 2019)

raw^dcp-1 has axon | third instar larval stage | conditional phenotype, suppressible | partially by Scer\GAL4^tey-5053A/kay^Fbz.UAS

(Hao et al., 2019)

raw^dcp-1 has embryonic/larval motor neuron | third instar larval stage | conditional phenotype, suppressible | partially by Scer\GAL4^tey-5053A/Jra^Jbz.UAS

(Hao et al., 2019)

raw^dcp-1 has embryonic/larval motor neuron | third instar larval stage | conditional phenotype, suppressible | partially by Scer\GAL4^tey-5053A/kay^Fbz.UAS

(Hao et al., 2019)

raw^dcp-1 has axon | third instar larval stage | conditional phenotype, suppressible | partially by hiw^ΔN

(Hao et al., 2019)

NOT suppressed by

Statement

Reference

raw^dcp-1 has axon | third instar larval stage | conditional phenotype, non-suppressible by Scer\GAL4^tey-5053A/Nmnat^GD8082

(Hao et al., 2019)

raw^dcp-1 has embryonic/larval motor neuron | third instar larval stage | conditional phenotype, non-suppressible by Scer\GAL4^tey-5053A/Nmnat^GD8082

(Hao et al., 2019)

raw^dcp-1 has axon | third instar larval stage | conditional phenotype, non-suppressible by Scer\GAL4^tey-5053A/bsk^K53R.UAS

(Hao et al., 2019)

raw^dcp-1 has embryonic/larval motor neuron | third instar larval stage | conditional phenotype, non-suppressible by Scer\GAL4^tey-5053A/bsk^K53R.UAS

(Hao et al., 2019)

NOT Enhancer of

Statement

Reference

raw^dcp-1/raw^134.47 is a non-enhancer of axon | larval stage phenotype of Hsap\SARM1^{SAM.TIR.UAS.Venus}, Scer\GAL4^tey-5053A

(Waller and Collins, 2023)

raw^dcp-1/raw^134.47 is a non-enhancer of embryonic/larval motor neuron | larval stage phenotype of Hsap\SARM1^{SAM.TIR.UAS.Venus}, Scer\GAL4^tey-5053A

(Waller and Collins, 2023)

NOT Suppressor of

Statement

Reference

raw^dcp-1/raw^134.47 is a non-suppressor of axon | larval stage phenotype of Hsap\SARM1^{SAM.TIR.UAS.Venus}, Scer\GAL4^tey-5053A

(Waller and Collins, 2023)

raw^dcp-1/raw^134.47 is a non-suppressor of embryonic/larval motor neuron | larval stage phenotype of Hsap\SARM1^{SAM.TIR.UAS.Venus}, Scer\GAL4^tey-5053A

(Waller and Collins, 2023)

Other

Statement

Reference

Scer\GAL4^tey-5053A, raw^dcp-1, wnd^GL00282 has axon | third instar larval stage phenotype

(Hao et al., 2019)

Scer\GAL4^tey-5053A, kay^Fbz.UAS, raw^dcp-1 has axon | third instar larval stage phenotype

(Hao et al., 2019)

Scer\GAL4^tey-5053A, kay^Fbz.UAS, raw^dcp-1 has embryonic/larval motor neuron | third instar larval stage phenotype

(Hao et al., 2019)

Scer\GAL4^tey-5053A, raw^dcp-1, wnd^GL00282 has embryonic/larval motor neuron | third instar larval stage phenotype

(Hao et al., 2019)

Scer\GAL4^tey-5053A, bsk^K53R.UAS, raw^dcp-1 has axon | third instar larval stage phenotype

(Hao et al., 2019)

Scer\GAL4^tey-5053A, bsk^K53R.UAS, raw^dcp-1 has embryonic/larval motor neuron | third instar larval stage phenotype

(Hao et al., 2019)

Scer\GAL4^tey-5053A, bsk^K53R.UAS, raw^dcp-1 has varicosity | ectopic | third instar larval stage | conditional phenotype

(Hao et al., 2019)

Jra^Jbz.UAS, Scer\GAL4^tey-5053A, raw^dcp-1 has axon | third instar larval stage phenotype

(Hao et al., 2019)

Jra^Jbz.UAS, Scer\GAL4^tey-5053A, raw^dcp-1 has embryonic/larval motor neuron | third instar larval stage phenotype

(Hao et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

raw^dcp-1 homozygotes show protection against (injury-induced) Wallerian degeneration in third instar larval motor neurons. This phenotype is considerably suppressed by hiw^ΔN or by the expression of either Jra^Jbz.UAS or kay^Fbz.UAS under the control of Scer\GAL4^tey-5053A; is only very slightly suppressed by the expression of Nmnat^GD8082 under the control of Scer\GAL4^tey-5053A (together with Dicer-2, for efficient RNAI); and is not significantly suppressed by the expression of bsk^K53R.UAS under the control of Scer\GAL4^tey-5053A.

In raw^dcp-1 homozygotes that also express either bsk^K53R.UAS, kay^Fbz.UAS, Jra^Jbz.UAS or wnd^GL00282 under the control of Scer\GAL4^tey-5053A, injured axons exhibit severely decreased new growth compared to controls. In the bsk^K53R.UAS setting, injured axons also develop varicosities.