FB2024_03 , released June 25, 2024
Allele: Dmel\sovFSTF-sfGFP
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General Information
Symbol
Dmel\sovFSTF-sfGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0356467
Feature type
allele
Associated gene
Dmel\sov
Associated Insertion(s)
Carried in Construct
PBac{FTSF.sfGFP-sov}
Key Links
Transgenic product class
genomic_DNA
(Benner et al., 2019)
wild_type
(Benner et al., 2019)
Mutagen
Nature of the Allele
Transgenic product class
genomic_DNA
(Benner et al., 2019)
wild_type
(Benner et al., 2019)
Mutagen
in vitro construct
(Benner et al., 2019)
Progenitor genotype
Carried in construct
PBac{FTSF.sfGFP-sov}
Cytology
Description

Genomic fragment (CH322-191E24 Pacman BAC clone) that encompasses sov. A 3xTag:FLAG-Tag:CS(TEVp)-Tag:StrepII-sfGFP-Tag:CALI(TC) cassette followed by a flexible 4xGlyGlySer linker has been inserted at the N-terminal end of the open reading frame.

(Benner et al., 2019)
Allele components
Component
Use(s)
Regulatory region(s)
sov
Encoded product / tool
sov
Tagged with
sfGFP
Tag:CALI(TC)
Tag:CS(TEVp)
Tag:FLAG
Tag:StrepII
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter or direct label
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      oogenesis
      germarium region 1
      (Benner et al., 2019)
      female germline stem cell
      (Benner et al., 2019)
      follicle cell
      (Benner et al., 2019)
      follicle stem cell
      (Benner et al., 2019)
      nurse cell
      (Benner et al., 2019)
      Additional Information
      Statement
      Reference

      sov protein is observed in the nucleus of germline cells within region 1 of the germarium, with the highest levels evident within the germline stem cells. Levels of sov are diminished in other areas of the germarium. sov is also observed within follicle cells and follicle stem cells. It is also observed in the nuclei of nurse cells and follicle cells in later-stage egg chambers.

      (Benner et al., 2019)
       
      Marker for
      Reflects expression of
      sov
      (Benner et al., 2019)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The lethality of Df(1)sov is rescued by sovFSTF-sfGFP.

      (Benner et al., 2019)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      sovFSTF-sfGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)