FB2024_03 , released June 25, 2024
Allele: Dmel\ash1ΔSET.Ubi-p63E
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General Information
Symbol
Dmel\ash1ΔSET.Ubi-p63E
Species
D. melanogaster
Name
FlyBase ID
FBal0351315
Feature type
allele
Associated gene
Dmel\ash1
Associated Insertion(s)
Carried in Construct
M{Ubi-p63E-ash1.ΔSET}
Key Links
Transgenic product class
inframe_deletion
(Dorafshan et al., 2019)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Dorafshan et al., 2019)
Mutagen
in vitro construct
(Dorafshan et al., 2019)
Progenitor genotype
Carried in construct
M{Ubi-p63E-ash1.ΔSET}
Cytology
Description

The Ubi-p63E promoter drives expression of a deleted ash1 open reading frame that lacks the SETdomain. The coding sequence is tagged with 'One-STrEP'.

(Dorafshan et al., 2019)
Allele components
Component
Use(s)
Regulatory region(s)
Ubi-p63E
Encoded product / tool
ash1
Tagged with
Tag:StrepII
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      adult abdomen, with Df(3L)Exel9011/ash13M, ash1ΔBAH.Ubi-p63E

      (Dorafshan et al., 2019)

      adult abdomen, with Df(3L)Exel9011/ash13M, ash1ΔPHD.Ubi-p63E

      (Dorafshan et al., 2019)

      adult abdominal segment 5, with Df(3L)Exel9011/ash122

      (Dorafshan et al., 2019)

      adult abdominal segment 6, with Df(3L)Exel9011/ash122

      (Dorafshan et al., 2019)

      adult mesothoracic segment, with Df(3L)Exel9011/ash13M, ash1ΔBAH.Ubi-p63E

      (Dorafshan et al., 2019)

      adult mesothoracic segment, with Df(3L)Exel9011/ash13M, ash1ΔPHD.Ubi-p63E

      (Dorafshan et al., 2019)

      adult mesothoracic segment, with Df(3L)Exel9011/ash122

      (Dorafshan et al., 2019)

      adult mesothoracic segment | ectopic, with Df(3L)Exel9011/ash13M, ash1ΔBAH.Ubi-p63E

      (Dorafshan et al., 2019)

      adult mesothoracic segment | ectopic, with Df(3L)Exel9011/ash13M, ash1ΔPHD.Ubi-p63E

      (Dorafshan et al., 2019)

      adult mesothoracic segment | ectopic, with Df(3L)Exel9011/ash122

      (Dorafshan et al., 2019)

      haltere, with Df(3L)Exel9011/ash13M, ash1ΔBAH.Ubi-p63E

      (Dorafshan et al., 2019)

      haltere, with Df(3L)Exel9011/ash13M, ash1ΔPHD.Ubi-p63E

      (Dorafshan et al., 2019)

      haltere, with Df(3L)Exel9011/ash122

      (Dorafshan et al., 2019)

      mesothoracic leg, with Df(3L)Exel9011/ash13M, ash1ΔBAH.Ubi-p63E

      (Dorafshan et al., 2019)

      mesothoracic leg, with Df(3L)Exel9011/ash13M, ash1ΔPHD.Ubi-p63E

      (Dorafshan et al., 2019)

      mesothoracic leg, with Df(3L)Exel9011/ash122

      (Dorafshan et al., 2019)

      mesothoracic tibial apical bristle, with Df(3L)Exel9011/ash122

      (Dorafshan et al., 2019)

      metathoracic bristle | ectopic, with Df(3L)Exel9011/ash122

      (Dorafshan et al., 2019)

      metathoracic leg | ectopic, with Df(3L)Exel9011/ash13M, ash1ΔBAH.Ubi-p63E

      (Dorafshan et al., 2019)

      metathoracic leg | ectopic, with Df(3L)Exel9011/ash13M, ash1ΔPHD.Ubi-p63E

      (Dorafshan et al., 2019)

      metathoracic leg | ectopic, with Df(3L)Exel9011/ash122

      (Dorafshan et al., 2019)

      wing | ectopic, with Df(3L)Exel9011/ash13M, ash1ΔBAH.Ubi-p63E

      (Dorafshan et al., 2019)

      wing | ectopic, with Df(3L)Exel9011/ash13M, ash1ΔPHD.Ubi-p63E

      (Dorafshan et al., 2019)

      wing | ectopic, with Df(3L)Exel9011/ash122

      (Dorafshan et al., 2019)

      wing sensillum | ectopic, with Df(3L)Exel9011/ash122

      (Dorafshan et al., 2019)
      Detailed Description
      Statement
      Reference

      ash1ΔSET.Ubi-p63E individuals do not show homeotic transformation.

      (Dorafshan et al., 2019)

      The ash122/Df(3L)Exel9011 individuals rescued to adulthood by ash1ΔSET.Ubi-p63E show homeotic transformation: transformation of the third thoracic segment (T3) to second thoracic segment (T2), including partial transformation of haltere to wing, characterized by the appearance of bristles on the haltere, appearance of hypopleural bristles on T3, and the third leg to second leg transformation manifested by the appearance of apical and pre-apical bristles on the third leg; transformation of posterior abdominal segments to more anterior fate, visible by the partial loss of pigmentation in tergites 5.

      The ash13M/Df(3L)Exel9011 individuals rescued to adulthood by ash1ΔPHD.Ubi-p63E/ash1ΔSET.Ubi-p63E or ash1ΔSET.Ubi-p63E/ash1ΔBAH.Ubi-p63E show milder or no homeotic transformation.

      (Dorafshan et al., 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      FBal0351315:, /Df(3L)Exel9011/ash122 is partially rescued by ash1ΔSET.Ubi-p63E/ash1ΔSET.Ubi-p63E

      (Dorafshan et al., 2019)

      FBal0351315:, /Df(3L)Exel9011/ash122 is partially rescued by ash1ΔSET.Ubi-p63E/ash1ΔSET.Ubi-p63E

      (Dorafshan et al., 2019)
      Not rescued by

      FBal0351315:, /Df(3L)Exel9011/ash13M is not rescued by ash1ΔSET.Ubi-p63E/ash1ΔSET.Ubi-p63E

      (Dorafshan et al., 2019)

      FBal0351315:, ash17F is not rescued by ash1ΔSET.Ubi-p63E/ash1ΔSET.Ubi-p63E

      (Dorafshan et al., 2019)
      Partially rescues

      ash1ΔSET.Ubi-p63E/ash1ΔSET.Ubi-p63E partially rescues FBal0351315:, /Df(3L)Exel9011/ash122

      (Dorafshan et al., 2019)

      ash1ΔSET.Ubi-p63E partially rescues Df(3L)Exel9011/ash122

      (Dorafshan et al., 2019)

      ash1ΔSET.Ubi-p63E/ash1ΔSET.Ubi-p63E partially rescues FBal0351315:, /Df(3L)Exel9011/ash122

      (Dorafshan et al., 2019)

      ash1ΔSET.Ubi-p63E/ash1ΔBAH.Ubi-p63E partially rescues Df(3L)Exel9011/ash13M

      (Dorafshan et al., 2019)

      ash1ΔSET.Ubi-p63E/ash1ΔPHD.Ubi-p63E partially rescues Df(3L)Exel9011/ash13M

      (Dorafshan et al., 2019)
      Fails to rescue

      ash1ΔSET.Ubi-p63E/ash1ΔSET.Ubi-p63E fails to rescue FBal0351315:, /Df(3L)Exel9011/ash13M

      (Dorafshan et al., 2019)

      ash1ΔSET.Ubi-p63E/ash1ΔSET.Ubi-p63E fails to rescue FBal0351315:, ash17F

      (Dorafshan et al., 2019)
      Comments

      ash1ΔSET.Ubi-p63E homozygosity rescues ash122/Df(3L)Exel9011 transheterozygotes to adulthood. However, adults have low fitness, with no stable stocks being established, and all individuals show homeotic transformation.

      (Dorafshan et al., 2019)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      ash1ΔSET.Ubi-p63E
      Name Synonyms
      Secondary FlyBase IDs
        References (2)