An indel (insertion of 3 nucleotides/deletion of 13 nucleotides) causing a frameshift at amino acid 42 that eventually truncates the protein to 115 amino acids. This truncated product lacks the last two conserved cysteines in the RING finger domain and therefore is likely nonfunctional.
Mutation causing a frameshift at amino acid 42 that eventually truncates the protein to 115 amino acids. This truncated product lacks the last two conserved cysteines in the RING finger domain and therefore is likely nonfunctional.
An indel (deletion of 16 nucleotides (ACTGCCCGCTATGCGG) and insertion of 3 nucleotides (GAA) at the same location) causes a frameshift at amino acid 42 that results in a protein of 115 amino acids. This truncated product lacks the last two conserved cysteines in the RING finger domain and therefore is likely nonfunctional.
GAA
naryaJJ6, nenyadel has abnormal meiotic cell cycle | female phenotype, suppressible by naryaUASp.GFP/Scer\GAL4VP16.nanos.UTR
Scer\GAL4VP16.nanos.UTR, naryaJJ6, nenyaRNAi.UAS has abnormal meiotic cell cycle | female phenotype
naryaJJ6, nenyadel has abnormal meiotic cell cycle | female phenotype
Scer\GAL4VP16.nanos.UTR, naryaJJ6, nenyaRNAi.UAS has germline cyst phenotype
Scer\GAL4VP16.nanos.UTR, naryaJJ6, nenyaRNAi.UAS has germarium region 2a phenotype
naryaJJ6 homozygous females that also express nenyadsRNA.UAS under the control of Scer\GAL4VP16.nos.UTR show frequent meiotic chromosome segregation errors, as assessed by X-chromosome non-disjunction; in agreement, meiotic recombination fails to initiate in early pachytene cysts, as few/no DSBs (i.e. γH2AV foci) and no crossovers along the X chromosome are detected. The oocyte is correctly specified by early-mid pachytene but does not show any obvious defects in karyosome structure. These females do not show changes in fertility, as compared to controls.
nenyadel/nenyadel, naryaJJ6/naryaJJ6 females show frequent meiotic chromosome segregation errors, as assessed by X-chromosome non-disjunction; in agreement, meiotic recombination fails to initiate in early pachytene cysts, with few if any DSBs being detected (γH2AV foci). The X-chromosome non-disjunction is fully rescued by the expression of naryaUASp.GFP under the control of Scer\GAL4VP16.nos.UTR. nenyadel/nenyadel, naryaJJ6/+ females, however, do not show significant meiotic chromosome segregation errors, as assessed by X-chromosome non-disjunction.
