GAL4::VP16 and a Disc\RFP3xP3.cUa marker flanked by loxP sites is inserted in Lkr upstream of the ATG (-111bp to -106bp, relative to start site).
ScerGAL4Lkr-GAL4::VP16 drives expression in muscles of the anterior hindgut and anal pad, the dorsal vessel and axons innervating it, sense organs of the proboscis, and muscles of the larval gastric caecum, midgut and hindgut.