FB2024_03 , released June 25, 2024
Allele: Dmel\Pi3K68DAH1
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General Information
Symbol
Dmel\Pi3K68DAH1
Species
D. melanogaster
Name
FlyBase ID
FBal0344929
Feature type
allele
Associated gene
Dmel\Pi3K68D
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Hauswirth et al., 2018)
Progenitor genotype
Cytology
Description

Indel mutation, premature stop codon at amino acid residue 1440.

(Hauswirth et al., 2018)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The neuromuscular junctions of third instar larval Pi3K68DAH1 mutants do not show significant changes in bouton or active zone density, as compared to controls. There is a significant decrease in EPSC and a small but significant decrease in quantal content, but no apparent changes in mEPSP and EPSP amplitudes, as compared to controls. Upon the expression of Pi3K68DUAS.EGFP under the control of Scer\GAL4C57, but not when under the control of Scer\GAL4VGlut-OK371, there is a decrease in mEPSP amplitude, as compared to controls.

      The third instar larval muscles of Pi3K68DAH1 mutants show significantly fewer recycling endosomes (Rab11-positive puncta) in the region adjacent to the synapse, but no changes in the number of late endosomes (Rab7-positive puncta), as compared to controls.

      (Hauswirth et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressor of
      Statement
      Reference

      Pi3K68DAH1 is a suppressor of abnormal neurophysiology | larval stage phenotype of GluRIIASP16

      (Hauswirth et al., 2018)
      Phenotype Manifest In
      Enhanced by
      Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Pi3K68DAH1
      (Hauswirth et al., 2018)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)