FlyBase Allele Report: Dmel\Lmx1a[Δ1]

General Information

Symbol

Dmel\Lmx1a^Δ1

Species

D. melanogaster

Name

FlyBase ID

FBal0343514

Feature type

allele

Associated gene

Dmel\Lmx1a

Associated Insertion(s)

TI{TI}Lmx1a^Δ1

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Allbee et al., 2018)

Mutagen

CRISPR/Cas9

(Allbee et al., 2018)

gene targeting by homologous recombination

(Allbee et al., 2018)

Progenitor genotype

Associated Insertion(s)

TI{TI}Lmx1a^Δ1

Cytology

Description

The Lmx1a open reading frame has been replaced by a Disc\RFP^{DsRed.3xP3.cUa} marker.

(Allbee et al., 2018)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | female (with Df(3L)BSC380)

(Allbee et al., 2018)

abnormal size | female (with Df(3L)ED4475)

(Allbee et al., 2018)

abnormal size | female (with Df(3L)ED4483)

(Allbee et al., 2018)

abnormal size | female | recessive

(Allbee et al., 2018)

abnormal size | pupal stage

(Allbee et al., 2018)

decreased fecundity | female | recessive

(Allbee et al., 2018)

female sterile | recessive

(Allbee et al., 2018)

male fertile

(Allbee et al., 2018)

viable

(Allbee et al., 2018)

viable (with Df(3L)BSC380)

(Allbee et al., 2018)

viable (with Df(3L)ED4475)

(Allbee et al., 2018)

viable (with Df(3L)ED4483)

(Allbee et al., 2018)

Phenotype Manifest In

ovarian sheath | adult stage

(Allbee et al., 2018)

ovariole | pupal stage

(Allbee et al., 2018)

ovary | adult stage

(Allbee et al., 2018)

ovary | adult stage (with Df(3L)BSC380)

(Allbee et al., 2018)

ovary | adult stage (with Df(3L)ED4475)

(Allbee et al., 2018)

ovary | adult stage (with Df(3L)ED4483)

(Allbee et al., 2018)

ovary | pupal stage

(Allbee et al., 2018)

terminal filament | adult stage

(Allbee et al., 2018)

terminal filament | late third instar larval stage

(Allbee et al., 2018)

terminal filament | pupal stage

(Allbee et al., 2018)

Detailed Description

Statement

Reference

Lmx1a^Δ1 homozygous and Lmx1a^Δ1/Df(3L)ED4475, Lmx1a^Δ1/Df(3L)ED4483 and Lmx1a^Δ1/Df(3L)BSC380 transheterozygous females show complete or near-complete loss of ovariole material/ovaries; Lmx1a^Δ1 homozygous females do not lay any eggs. In limited genetic backgrounds, including a non-backcross Lmx1a^Δ1/Tm3 balanced stock, residual ovary tissue can be seen in newly eclosed virgin Lmx1a^Δ1 homozygous females but without morphologically recognizable terminal filaments.

Early at the larval-pupal transition, Lmx1a^Δ1 ovaries show partial evidence of terminal filament morphogenesis but these do not form the collection of fully formed, linear stacks; by 24h/48h after puparium formation, terminal filaments are absent, ovaries show no sign of growth and elongation, and primordial ovarioles do not form.

(Allbee et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Lmx1a^Δ1 has decreased fecundity | recessive | female phenotype, suppressible | partially by Scer\GAL4^GMR42B09/Ggal\LMX1B^UAS.cAa

(Allbee et al., 2018)

Lmx1a^Δ1 has abnormal size | recessive | female phenotype, suppressible | partially by Scer\GAL4^GMR42B09/Ggal\LMX1B^UAS.cAa

(Allbee et al., 2018)

Phenotype Manifest In

Suppressed by

Statement

Reference

Lmx1a^Δ1 has ovary | adult stage phenotype, suppressible | partially by Scer\GAL4^GMR42B09/Ggal\LMX1B^UAS.cAa

(Allbee et al., 2018)

Lmx1a^Δ1 has terminal filament | adult stage phenotype, suppressible | partially by Scer\GAL4^GMR42B09/Ggal\LMX1B^UAS.cAa

(Allbee et al., 2018)

Lmx1a^Δ1 has ovarian sheath | adult stage phenotype, suppressible | partially by Scer\GAL4^GMR42B09/Ggal\LMX1B^UAS.cAa

(Allbee et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Lmx1a^Δ1 is partially rescued by Scer\GAL4^GMR42B09/Lmx1a^{UAS.ORF.GW.Tag:HA}

(Allbee et al., 2018)

Comments

Expressing Lmx1a^{UAS.ORF.GW.Tag:HA} under teh control of Scer\GAL4^GMR42B09 significantly restores overall ovary morphogenesis, including sheath development, TF formation and egg laying in Lmx1a^Δ1 homozygous females. Ovary morphology is also partially rescued if this expression is only induced from mid-L3 but not from 2h after pupariation (using Gal80[ts] to temporally control the expression).

(Allbee et al., 2018)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Lmx1a^Δ1

(Allbee et al., 2018)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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