FlyBase Allele Report: Dmel\ash1[R1288A]

General Information

Symbol

Dmel\ash1^R1288A

Species

D. melanogaster

Name

FlyBase ID

FBal0337864

Feature type

allele

Associated gene

Dmel\ash1

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Huang et al., 2017)

Progenitor genotype

Cytology

Description

Amino acid replacement: R1288A.

(Huang et al., 2017)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

MNV

3L:19,594,311..19,594,313 [-]

Amino acid change:

R1288A | ash1-PB; R1288A | ash1-PC

Reported amino acid change:

R1288A

Comment:

CRISPR/Cas9 replacement of codon 1288 from R (CGA) to A (GCT).

(Huang et al., 2017)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

homeotic (with ash1²²)

(Huang et al., 2017)

homeotic | recessive

(Huang et al., 2017)

visible (with ash1²²)

(Huang et al., 2017)

visible | recessive

(Huang et al., 2017)

Phenotype Manifest In

adult sensillum

(Huang et al., 2017)

adult sensillum (with ash1²²)

(Huang et al., 2017)

haltere pedicel | adult stage

(Huang et al., 2017)

haltere pedicel | adult stage (with ash1²²)

(Huang et al., 2017)

leg sensillum | adult stage | ectopic

(Huang et al., 2017)

leg sensillum | adult stage | ectopic (with ash1²²)

(Huang et al., 2017)

metathoracic leg | adult stage

(Huang et al., 2017)

metathoracic leg | adult stage (with ash1²²)

(Huang et al., 2017)

Detailed Description

Statement

Reference

Most ash1^R1288A/ash1²² transheterozygotes and ash1^R1288A homozygotes exhibit a third leg to second leg partial transformation, as indicated by the presence of ectopic apical bristles, as compared to wild-type and heterozygous controls. Most of ash1^R1288A/ash1²² transheterozygotes and a small proportion ash1^R1288A homozygotes show a haltere to wing partial transformation, as indicated by the presence of ectopic bristles on the pedicel, as compared to wild-type and heterozygous controls.

(Huang et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ash1^R1288A has visible | recessive phenotype, suppressible by Df(2R)XL26/+

(Huang et al., 2017)

ash1^R1288A has homeotic | recessive phenotype, suppressible by Df(2R)XL26/+

(Huang et al., 2017)

ash1^R1288A/ash1²² has visible phenotype, suppressible by Df(2R)XL26/+

(Huang et al., 2017)

ash1^R1288A/ash1²² has homeotic phenotype, suppressible by Df(2R)XL26/+

(Huang et al., 2017)

ash1^R1288A/ash1²² has visible phenotype, suppressible | partially by Scer\GAL4^arm.PS/Caf1-55::MRG15^UAS.cHa

(Huang et al., 2017)

ash1^R1288A/ash1²² has homeotic phenotype, suppressible | partially by Scer\GAL4^arm.PS/Caf1-55::MRG15^UAS.cHa

(Huang et al., 2017)

ash1^R1288A/ash1²² has visible phenotype, suppressible | partially by Scer\GAL4^arm.PS/Caf1-55::MRG15^Δchromo.UAS

(Huang et al., 2017)

ash1^R1288A/ash1²² has homeotic phenotype, suppressible | partially by Scer\GAL4^arm.PS/Caf1-55::MRG15^Δchromo.UAS

(Huang et al., 2017)

ash1^R1288A has visible | recessive phenotype, suppressible | partially by Scer\GAL4^arm.PS/Caf1-55::MRG15^UAS.cHa

(Huang et al., 2017)

ash1^R1288A has homeotic | recessive phenotype, suppressible | partially by Scer\GAL4^arm.PS/Caf1-55::MRG15^UAS.cHa

(Huang et al., 2017)

ash1^R1288A/ash1²² has visible phenotype, suppressible | partially by Caf1-55::Hsap\MORF4L1^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

ash1^R1288A/ash1²² has homeotic phenotype, suppressible | partially by Caf1-55::Hsap\MORF4L1^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

NOT suppressed by

Statement

Reference

ash1^R1288A/ash1²² has visible phenotype, non-suppressible by MRG15^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

ash1^R1288A/ash1²² has homeotic phenotype, non-suppressible by MRG15^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

ash1^R1288A/ash1²² has visible phenotype, non-suppressible by Caf1-55^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

ash1^R1288A/ash1²² has homeotic phenotype, non-suppressible by Caf1-55^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

ash1^R1288A/ash1²² has visible phenotype, non-suppressible | partially by Scer\GAL4^arm.PS/Caf1-55::MRG15^UAS.cHa

(Huang et al., 2017)

ash1^R1288A/ash1²² has homeotic phenotype, non-suppressible | partially by Scer\GAL4^arm.PS/Caf1-55::MRG15^UAS.cHa

(Huang et al., 2017)

ash1^R1288A has visible | recessive phenotype, non-suppressible by MRG15^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

ash1^R1288A has homeotic | recessive phenotype, non-suppressible by MRG15^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

ash1^R1288A has visible | recessive phenotype, non-suppressible by Caf1-55^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

ash1^R1288A has homeotic | recessive phenotype, non-suppressible by Caf1-55^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

ash1^R1288A has metathoracic leg | adult stage phenotype, suppressible by Df(2R)XL26/+

(Huang et al., 2017)

ash1^R1288A has leg sensillum | ectopic | adult stage phenotype, suppressible by Df(2R)XL26/+

(Huang et al., 2017)

ash1^R1288A/ash1²² has haltere pedicel | adult stage phenotype, suppressible by Df(2R)XL26/+

(Huang et al., 2017)

ash1^R1288A/ash1²² has adult sensillum phenotype, suppressible by Df(2R)XL26/+

(Huang et al., 2017)

ash1^R1288A/ash1²² has haltere pedicel | adult stage phenotype, suppressible | partially by Scer\GAL4^arm.PS/Caf1-55::MRG15^UAS.cHa

(Huang et al., 2017)

ash1^R1288A/ash1²² has adult sensillum phenotype, suppressible | partially by Scer\GAL4^arm.PS/Caf1-55::MRG15^UAS.cHa

(Huang et al., 2017)

ash1^R1288A/ash1²² has haltere pedicel | adult stage phenotype, suppressible | partially by Scer\GAL4^arm.PS/Caf1-55::MRG15^Δchromo.UAS

(Huang et al., 2017)

ash1^R1288A/ash1²² has adult sensillum phenotype, suppressible | partially by Scer\GAL4^arm.PS/Caf1-55::MRG15^Δchromo.UAS

(Huang et al., 2017)

ash1^R1288A has metathoracic leg | adult stage phenotype, suppressible | partially by Scer\GAL4^arm.PS/Caf1-55::MRG15^UAS.cHa

(Huang et al., 2017)

ash1^R1288A has leg sensillum | ectopic | adult stage phenotype, suppressible | partially by Scer\GAL4^arm.PS/Caf1-55::MRG15^UAS.cHa

(Huang et al., 2017)

ash1^R1288A has leg sensillum | ectopic | adult stage phenotype, suppressible | partially by Scer\GAL4^arm.PS/Caf1-55::MRG15^Δchromo.UAS

(Huang et al., 2017)

ash1^R1288A/ash1²² has haltere pedicel | adult stage phenotype, suppressible | partially by Caf1-55::Hsap\MORF4L1^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

ash1^R1288A/ash1²² has adult sensillum phenotype, suppressible | partially by Caf1-55::Hsap\MORF4L1^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

NOT suppressed by

Statement

Reference

ash1^R1288A/ash1²² has haltere pedicel | adult stage phenotype, non-suppressible by MRG15^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

ash1^R1288A/ash1²² has adult sensillum phenotype, non-suppressible by MRG15^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

ash1^R1288A/ash1²² has haltere pedicel | adult stage phenotype, non-suppressible by Caf1-55^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

ash1^R1288A/ash1²² has adult sensillum phenotype, non-suppressible by Caf1-55^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

ash1^R1288A has metathoracic leg | adult stage phenotype, non-suppressible by MRG15^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

ash1^R1288A has leg sensillum | ectopic | adult stage phenotype, non-suppressible by MRG15^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

ash1^R1288A has metathoracic leg | adult stage phenotype, non-suppressible by Caf1-55^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

ash1^R1288A has leg sensillum | ectopic | adult stage phenotype, non-suppressible by Caf1-55^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

ash1^R1288A/ash1²² has metathoracic leg | adult stage phenotype, non-suppressible by Scer\GAL4^arm.PS/Caf1-55::MRG15^UAS.cHa

(Huang et al., 2017)

ash1^R1288A/ash1²² has leg sensillum | ectopic | adult stage phenotype, non-suppressible by Scer\GAL4^arm.PS/Caf1-55::MRG15^UAS.cHa

(Huang et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The haltere to wing partial transformation exhibited by most ash1^R1288A/ash1²² transheterozygotes is fully suppressed by one copy of Df(2R)XL26 and is partially suppressed by the expression of Caf1-55::MRG15^Scer\UAS.cHa or Caf1-55::MRG15^{Δchromo.Scer\UAS}, but not of MRG15^Scer\UAS.cHa or Caf1-55^Scer\UAS.cHa, under the control of Scer\GAL4^arm.PS; their third leg to second leg partial transformation, however, is not suppressed by the expression of Caf1-55::MRG15^Scer\UAS.cHa under the control of Scer\GAL4^arm.PS.

The third leg to second leg partial transformation exhibited by most ash1^R1288A homozygotes is fully suppressed by one copy of Df(2R)XL26 and is partially suppressed by the expression of Caf1-55::MRG15^Scer\UAS.cHa, but not MRG15^Scer\UAS.cHa or Caf1-55^Scer\UAS.cHa, under the control of Scer\GAL4^arm.PS.

(Huang et al., 2017)

Xenogenetic Interactions

Statement

Reference

(Huang et al., 2017)

Complementation and Rescue Data

Rescued by

ash1^R1288A is rescued by ash1^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

ash1^R1288A/ash1²² is rescued by ash1^UAS.cHa/Scer\GAL4^arm.PS

(Huang et al., 2017)

Comments

Both the third leg to second leg partial transformation exhibited by both ash1^R1288A/ash1²² transheterozygotes and ash1^R1288A homozygotes and the haltere to wing partial transformation exhibited by ash1^R1288A/ash1²² transheterozygotes are rescued by the expression of ash1^Scer\UAS.cHa under the control of Scer\GAL4^arm.PS.