FlyBase Allele Report: Dmel\barc[RNAi.UAS]

General Information

Symbol

Dmel\barc^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0337820

Feature type

allele

Associated gene

Dmel\barc

Associated Insertion(s)

Carried in Construct

P{UAS-barc.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Abramczuk et al., 2017)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Abramczuk et al., 2017)

Mutagen

in vitro construct

(Abramczuk et al., 2017)

Progenitor genotype

Carried in construct

P{UAS-barc.RNAi}

Cytology

Description

UAS regulatory sequences (derived from the pValium20 plasmid) drive expression of an shRNA that targets barc.

(Abramczuk et al., 2017)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

barc

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number, with Scer\GAL4^insc-Mz1407

(Abramczuk et al., 2017)

abnormal neuroanatomy, with Scer\GAL4^ase.neuro

(Abramczuk et al., 2017)

abnormal neuroanatomy, with Scer\GAL4^insc-Mz1407

(Abramczuk et al., 2017)

increased cell number, with Scer\GAL4^ase.neuro

(Abramczuk et al., 2017)

Phenotype Manifest In

ganglion mother cell, with Scer\GAL4^ase.neuro

(Abramczuk et al., 2017)

ganglion mother cell, with Scer\GAL4^insc-Mz1407

(Abramczuk et al., 2017)

intermediate neuronal progenitor, with Scer\GAL4^insc-Mz1407

(Abramczuk et al., 2017)

neuron, with Scer\GAL4^insc-Mz1407

(Abramczuk et al., 2017)

Detailed Description

Statement

Reference

The expression of barc^{shRNA.Scer\UAS} under the control of Scer\GAL4^insc-Mz1407 leads to a significant increase in the number of intermediate neural progenitors, namely of immature Ase-positive, Dpn-negative intermediates, and to significant decreases in the numbers of ganglion mother cells and of Pros-positive neurons in type II neuroblast lineages, as compared to controls. Expression under the control of either Scer\GAL4^ase.neuro or Scer\GAL4^insc-Mz1407 leads to a significant increase in the number of ganglion mother cells in type I neuroblast lineages, as compared to controls.

(Abramczuk et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^insc-Mz1407, barc^RNAi.UAS has abnormal cell number phenotype, suppressible by Mmus\Htatsf1^UAS.Tag:MYC, Scer\GAL4^insc-Mz1407

(Abramczuk et al., 2017)

Scer\GAL4^ase.neuro, barc^RNAi.UAS has increased cell number phenotype, suppressible by Mmus\Htatsf1^UAS.Tag:MYC, Scer\GAL4^ase.neuro

(Abramczuk et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^insc-Mz1407, barc^RNAi.UAS has intermediate neuronal progenitor phenotype, suppressible by Mmus\Htatsf1^UAS.Tag:MYC, Scer\GAL4^insc-Mz1407

(Abramczuk et al., 2017)

Scer\GAL4^insc-Mz1407, barc^RNAi.UAS has ganglion mother cell phenotype, suppressible by Mmus\Htatsf1^UAS.Tag:MYC, Scer\GAL4^insc-Mz1407

(Abramczuk et al., 2017)

Scer\GAL4^insc-Mz1407, barc^RNAi.UAS has neuron phenotype, suppressible by Mmus\Htatsf1^UAS.Tag:MYC, Scer\GAL4^insc-Mz1407

(Abramczuk et al., 2017)

Scer\GAL4^ase.neuro, barc^RNAi.UAS has ganglion mother cell phenotype, suppressible by Mmus\Htatsf1^UAS.Tag:MYC, Scer\GAL4^ase.neuro

(Abramczuk et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

(Abramczuk et al., 2017)

Complementation and Rescue Data

Rescued by

barc^RNAi.UAS is rescued by Scer\GAL4^insc-Mz1407/barc^{UAS.RR.Tag:MYC}

(Abramczuk et al., 2017)

barc^RNAi.UAS is rescued by Scer\GAL4^ase.neuro/barc^{UAS.RR.Tag:MYC}

(Abramczuk et al., 2017)

Not rescued by

barc^RNAi.UAS is not rescued by Scer\GAL4^insc-Mz1407/barc^{Y287D.UAS.RR.Tag:MYC}

(Abramczuk et al., 2017)

Comments

Both the type I and type II neuroblast lineage phenotypes induced by the expression of barc^{shRNA.Scer\UAS} under the control of Scer\GAL4^ase.neuro or Scer\GAL4^insc-Mz1407 are rescued by the co-expression of barc^{Scer\UAS.RNAi-res.T:Hsap\MYC}; the type II neuroblast lineage phenotypes, however, are not rescued by the co-expression of barc^{Y287D.Scer\UAS.RNAi-res.T:Hsap\MYC}.

(Abramczuk et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

barc^RNAi.UAS

barc^{shRNA.Scer\UAS}

barc^shRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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