FlyBase Allele Report: Dmel\kud[KO]

General Information

Symbol

Dmel\kud^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0335708

Feature type

allele

Associated gene

Dmel\kud

Associated Insertion(s)

TI{TI}kud^KO

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Ding et al., 2017)

Mutagen

gene targeting by homologous recombination

(Ding et al., 2017)

I-SceI

(Ding et al., 2017)

FLPase

(Ding et al., 2017)

Progenitor genotype

Associated Insertion(s)

TI{TI}kud^KO

Cytology

Description

The coding sequence of kud plus its 5' and 3' UTRs are replaced with the w gene and two loxP sites from donor DNA.

(Ding et al., 2017)

Allele components

Component

Use(s)

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

3L:16,844,577..16,845,002 [-]

Comment:

The knockout mutation was created by replacing the coding sequence and 5' and 3' UTRs of kud with the w gene and two loxP sites.

(Ding and Huang, 2018.2.21)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body size | larval stage

(Ding et al., 2017)

decreased cell size | oogenesis | somatic clone

(Ding et al., 2017)

increased cell death | oogenesis | somatic clone

(Ding et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

5 days old kud^KO homozygous larvae display a severely reduced body size, as compared to age-matched controls. kud^KO homozygous follicular epithelium clones exhibit a strong decrease in average transverse cell area, a severe increase in the frequency of clones exhibiting apoptotic cells (labelled by activated caspase 3), and reduced level of lysosomes/autolysosomes (labeled by Lysotracker), as compared to controls.

kud^KO homozygous and kud^KO/Df(3L)Exel9002 transheterozygous third instar larvae exhibit autophagy defects, shown by reduced levels of Atg8a protein in Western blots, as compared to controls.

Third instar larval muscle fibers from individuals only heterozygous for kud^KO or also expressing kud^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Mef2.247 exhibit mild and moderate nuclear spacing defects (i.e. nuclear clustering), respectively, but not nuclear shape defects, as compared to controls.

(Ding et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

kud^KO has increased cell death | oogenesis | somatic clone phenotype, enhanceable by klar^{KASH.UAS.Tag:HA}/Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Ding et al., 2017)

kud^KO has increased cell death | oogenesis | somatic clone phenotype, enhanceable by klar^{5'ΔA.UAS.6.Tag:MYC}/Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Ding et al., 2017)

Suppressed by

Statement

Reference

kud^KO has increased cell death | somatic clone | oogenesis phenotype, suppressible | partially by koi[+]/koi⁸⁰

(Ding et al., 2017)

kud^KO has decreased cell size | somatic clone | oogenesis phenotype, suppressible | partially by klar^Δ1-18/klar^Δ1-18

(Ding et al., 2017)

kud^KO has increased cell death | somatic clone | oogenesis phenotype, suppressible by Hsap\TMEM258^UAS.CFP/Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Ding et al., 2017)

kud^KO has increased cell death | somatic clone | oogenesis phenotype, suppressible | partially by Msp300^compl/Msp300[+]

(Ding et al., 2017)

kud^KO has increased cell death | somatic clone | oogenesis phenotype, suppressible | partially by klar^Δ1-18/klar^Δ1-18

(Ding et al., 2017)

kud^KO has increased cell death | somatic clone | oogenesis phenotype, suppressible | partially by klar^{3'ΔS.UAS.6.Tag:MYC}/Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Ding et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

kud^KO has follicle cell | somatic clone | oogenesis phenotype, suppressible | partially by Msp300^compl/Msp300[+]

(Ding et al., 2017)

kud^KO has follicle cell | somatic clone | oogenesis phenotype, suppressible | partially by klar^Δ1-18/klar^Δ1-18

(Ding et al., 2017)

kud^KO has follicle cell | somatic clone | oogenesis phenotype, suppressible | partially by klar^{3'ΔS.UAS.6.Tag:MYC}/Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Ding et al., 2017)

kud^KO has follicle cell | somatic clone | oogenesis phenotype, suppressible | partially by koi[+]/koi⁸⁰

(Ding et al., 2017)

kud^KO has follicle cell | somatic clone | oogenesis phenotype, suppressible | partially by klar^{KASH.UAS.Tag:HA}/Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Ding et al., 2017)

kud^KO has follicle cell | somatic clone | oogenesis phenotype, suppressible | partially by klar^{5'ΔA.UAS.6.Tag:MYC}/Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Ding et al., 2017)

kud^KO has follicle cell | somatic clone | oogenesis phenotype, suppressible by Hsap\TMEM258^UAS.CFP/Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Ding et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The increased apoptosis observed in kud^KO homozygous follicular epithelium clones is partially suppressed by heterozygosity for Msp300^compl or koi⁸⁰, by homozygosity for klar^Δ1-18, or by the expression of klar^{3'ΔS.Scer\UAS.6.T:Hsap\MYC} under the control of Scer\GAL4^{Scer\FRT.Rnor\Cd2.Act5C}, while being enhanced by the expression of klar^{Scer\UAS.T:Ivir\HA1} or klar^{5'ΔA.Scer\UAS.6.T:Hsap\MYC} under the control of Scer\GAL4^{Scer\FRT.Rnor\Cd2.Act5C}. The decreased transverse cell areas in kud^KO homozygous follicular epithelium clones are also partially suppressed by homozygosity for klar^Δ1-18.

(Ding et al., 2017)

Xenogenetic Interactions

Statement

Reference

The increased apoptosis observed in kud^KO homozygous follicular epithelium clones are suppressed by the expression of Hsap\TMEM258^{Scer\UAS.T:Avic\GFP-CFP} under the control of Scer\GAL4^{Scer\FRT.Rnor\Cd2.Act5C}.

(Ding et al., 2017)

Complementation and Rescue Data

Rescued by

kud^KO is rescued by kud^UAS.CFP/Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Ding et al., 2017)

kud^KO is rescued by kud^UAS.GFP/Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Ding et al., 2017)

kud^KO is rescued by kud^{ΔN.UAS.Tag:HA}/Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Ding et al., 2017)

Not rescued by

kud^KO is not rescued by Scer\GAL4^{FRT.Rnor\Cd2.Act5C}/kud^IM-J.UAS.GFP

(Ding et al., 2017)