The VT044845 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).
Comment: when combined with P{VT037220-p65.AD}
when combined with P{VT050660-p65.AD} (combination referred to as 'MDN-1-GAL4')
when combined with P{VT050660-p65.AD} (combination referred to as 'MDN3-GAL4')
when combined with P{VT050660-p65.AD}
Thermogenetic activation (using TrpA1UAS.cvPa at 30[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.VT044845 and Hsap\RELAAD.VT050660, or the split-GAL4 pair of Scer\GAL4DBD.VT044845 and Hsap\RELAAD.VT037220, results in a significant increase in the total distance and bout distance of backwards walking, with a significant decrease in forwards walking, compared to controls.
Inhibition (using Ctet\TeTxLCTNT.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.VT044845 and Hsap\RELAAD.VT050660, or the split-GAL4 pair of Scer\GAL4DBD.VT044845 and Hsap\RELAAD.VT037220, significantly reduces backing-up behavior when adult flies reach a narrow dead end, compared to controls; in the case of Scer\GAL4DBD.VT044845 and Hsap\RELAAD.VT037220, but not Scer\GAL4DBD.VT044845 and Hsap\RELAAD.VT050660; there is a small, significant decrease in total forwards walking distance in unimpeded conditions, compared to controls.