FB2024_03 , released June 25, 2024
Allele: Hsap\RELAAD.VT037220
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General Information
Symbol
Hsap\RELAAD.VT037220
Species
H. sapiens
Name
FlyBase ID
FBal0330486
Feature type
allele
Associated gene
Hsap\RELA
Associated Insertion(s)
Carried in Construct
P{VT037220-p65.AD}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Rubin Laboratory at Janelia Farm, 2017)
Progenitor genotype
Carried in construct
P{VT037220-p65.AD}
Cytology
Description

The VT037220 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).

(Rubin Laboratory at Janelia Farm, 2017)
Allele components
Component
Use(s)
Regulatory region(s)
VT037220
Encoded product / tool
p65(AD)::Zip+
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult brain | restricted

      Comment: when combined with P{VT044845-GAL4.DBD}

      (Bidaye et al., 2014)
      adult moonwalker descending neuron

      Comment: when combined with P{VT044845-GAL4.DBD}

      (Bidaye et al., 2014)
      adult ventral nerve cord | restricted

      Comment: when combined with P{VT044845-GAL4.DBD}

      (Bidaye et al., 2014)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Thermogenetic activation (using TrpA1UAS.cvPa at 30[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.VT044845 and Hsap\RELAAD.VT037220 results in a significant increase in the total distance and bout distance of backwards walking, with a significant decrease in forwards walking, compared to controls.

      Inhibition (using Ctet\TeTxLCTNT.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.VT044845 and Hsap\RELAAD.VT037220 significantly reduces backing-up behavior when adult flies reach a narrow dead end, compared to controls; there is a small, significant decrease in total forwards walking distance in unimpeded conditions, compared to controls.

      (Bidaye et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hsap\RELAAD.VT037220.T:Zzzz\Zip+
      Hsap\RELAAD.VT037220
      Name Synonyms
      Secondary FlyBase IDs
        References (4)