FB2024_03 , released June 25, 2024
Allele: Dmel\Mst77FΔ1
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General Information
Symbol
Dmel\Mst77FΔ1
Species
D. melanogaster
Name
FlyBase ID
FBal0326352
Feature type
allele
Associated gene
Dmel\Mst77F
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Kimura and Loppin, 2016)
Mutagen
CRISPR/Cas9
(Kimura and Loppin, 2016)
Progenitor genotype
Cytology
Description

4bp deletion within the Mst77F coding sequence.

(Kimura and Loppin, 2016)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3L:20,817,848..20,817,851 [-]
Comment:

4bp deletion within the Mst77F coding sequence starting at amino acid 10.

(Kimura and Loppin, 2016)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Mst77FΔ1 homozygotes are male sterile; Mst77FΔ1/Mst77FΔ2, Mst77FΔ1/Mst77FΔ3 and Mst77FΔ1/Df(3L)BSC452 transheterozygous males produce very rare progeny; Mst77FΔ1/Df(3L)BSC452 transheterozygous males and Mst77FΔ1/Df(3L)ri-79c transheterozygous males are fertile but display reduced fertility, as compared to controls.

      Mst77FΔ1 homozygous adult males present smaller seminal vesicles, which do not contain mature sperm, as compared to controls; Mst77FΔ1 homozygotes and Mst77FΔ1/Mst77FΔ2 or Mst77FΔ1/Df(3L)BSC452 transheterozygotes present defective spermatid individualization, as compared to controls: spermatids are in disorganized bundles, retain the individualization complexes and present defects in nuclei morphology and chromatin compaction, as compared to controls.

      Mst77FΔ1/Mst77FΔ2 and Mst77FΔ1/Df(3L)BSC452 transheterozygotes occasionally present post-individualization spermatid bundles without obvious morphology defects, as compared to controls.

      (Kimura and Loppin, 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Enhanced by
      NOT suppressed by
      Statement
      Reference

      Mst77FΔ1 has spermatid cyst | spermatogenesis phenotype, non-suppressible by ProtB[+]/ProtBEGFP

      (Kimura and Loppin, 2016)

      Mst77FΔ1 has individualization stage spermatid | spermatogenesis phenotype, non-suppressible by ProtB[+]/ProtBEGFP

      (Kimura and Loppin, 2016)

      Mst77FΔ1 has cystic bulge | spermatogenesis phenotype, non-suppressible by ProtB[+]/ProtBEGFP

      (Kimura and Loppin, 2016)

      Mst77FΔ1 has nucleus | spermatogenesis phenotype, non-suppressible by ProtB[+]/ProtBEGFP

      (Kimura and Loppin, 2016)

      Mst77FΔ1 has chromatin | spermatogenesis phenotype, non-suppressible by ProtB[+]/ProtBEGFP

      (Kimura and Loppin, 2016)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Mst77FΔ1, PBac{PB}Pka-R1c06969 and Mst77FΔ1, Df(2L)ΔMst35B double heterozygous males are fertile, but display a small reduction in fertility as compared to controls.

      Mst77FΔ1/+, Df(2L)ΔMst35B/Df(2L)ΔMst35B males exhibit near sterility and defective spermatid individualization, as compared to controls: post-individualization spermatids are in disorganized bundles and exhibit defects in nuclei morphology and chromatin compaction. All these defects are rescued by one copy of Mst77F+tg.

      The spermatogenesis defects exhibited by Mst77FΔ1 homozygotes are not enhanced by Df(2L)ΔMst35B heterozygosity or homozygosity, nor suppressed by one copy of ProtBT:Avic\GFP-EGFP.

      (Kimura and Loppin, 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      Mst77FΔ3

      (Kimura and Loppin, 2016)

      Mst77FΔ2

      (Kimura and Loppin, 2016)
      Partially rescued by

      Mst77FΔ1 is partially rescued by Mst77F+tg

      (Kimura and Loppin, 2016)

      Mst77FΔ1/Mst77FΔ2 is partially rescued by Mst77F+tg

      (Kimura and Loppin, 2016)

      Mst77FΔ1/Mst77FΔ2 is partially rescued by Mst77FmRFP1

      (Kimura and Loppin, 2016)

      Mst77FΔ1/Df(3L)BSC452 is partially rescued by Mst77F+tg

      (Kimura and Loppin, 2016)
      Not rescued by

      Mst77FΔ1/Mst77FΔ2 is not rescued by Mst77FEGFP

      (Kimura and Loppin, 2016)
      Comments

      Mst77FΔ1 fails to complement either Mst77FΔ2 or Mst77F[Δ3].

      The male sterility of Mst77FΔ1 homozygotes and the male semi-sterility of Mst77FΔ1/Df(3L)BSC452 or Mst77FΔ1/Mst77FΔ2 heterozygotes are partially rescued by one copy of Mst77F+tg; the male semi-sterility observed in Mst77FΔ1/Mst77FΔ2 transheterozygotes is also partially rescued by one copy of Mst77FT:Disc\RFP-mRFP, but not of Mst77FT:Avic\GFP-EGFP.

      The spermatogenesis defects observed in Mst77FΔ1 homozygotes are also partially rescued by one copy of Mst77F+tg.

      (Kimura and Loppin, 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Mst77F (Δ1)
      (Chang et al., 2023)
      Mst77FΔ1
      (Kimura and Loppin, 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)