FlyBase Allele Report: Dmel\clu[ΔW]

General Information

Symbol

Dmel\clu^ΔW

Species

D. melanogaster

Name

FlyBase ID

FBal0325678

Feature type

allele

Associated gene

Dmel\clu

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Wang et al., 2015)

Progenitor genotype

P{SUPor-P}clu^KG02346

(Wang et al., 2015)

Cytology

Description

Deletion that removes the 5'UTR and start codon of clu. Generated by imprecise excision of P{SUPor-P}clu^KG02346.

(Wang et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | third instar larval stage

(Wang et al., 2016)

abnormal locomotor behavior | third instar larval stage (with clu^d08713)

(Wang et al., 2016)

partially lethal - majority die

(Wang et al., 2016)

Phenotype Manifest In

mitochondrial crista | third instar larval stage

(Wang et al., 2016)

mitochondrion | third instar larval stage

(Wang et al., 2016)

mitochondrion | third instar larval stage (with clu^d08713)

(Wang et al., 2016)

Detailed Description

Statement

Reference

clu^ΔW homozygous as well as clu^ΔW/clu^d08713 transheterozygous mutant third instar larvae display mitochondrial morphology defects - clustered, rounded mitochondria with disrupted cristae - in larval muscles and these larvae also display significant locomotion deficit (reduced crawling speed).

Indirect flight muscles in 4-day-old clu^ΔW/clu^d08713 adults are not apoptotic (assessed by TUNEL assay).

(Wang et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

clu^ΔW has abnormal locomotor behavior | third instar larval stage phenotype, non-enhanceable by park¹/park¹

(Wang et al., 2016)

NOT Enhancer of

Statement

Reference

clu^ΔW/clu^ΔW is a non-enhancer of abnormal locomotor behavior | third instar larval stage phenotype of park¹

(Wang et al., 2016)

Other

Statement

Reference

clu^ΔW, park¹ has lethal phenotype

(Wang et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

clu^ΔW has mitochondrion | third instar larval stage phenotype, enhanceable by park¹/park¹

(Wang et al., 2016)

Enhancer of

Statement

Reference

clu^ΔW/clu^ΔW is an enhancer of mitochondrion | third instar larval stage phenotype of park¹

(Wang et al., 2016)

Other

Statement

Reference

clu^ΔW, park¹/park[+] has mitochondrion | third instar larval stage phenotype

(Wang et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

clu^ΔW/+;park¹/+ double heterozygous larvae show abnormal clustering of mitochondria around nuclei in muscle cells that is not observed in either of the single heterozygotes.

The mitochondrial morphology defects observed in larval muscles of both clu^ΔW and park¹ homozygotes are exacerbated further in the double mutants, moreover while either clu^ΔW or park¹ are partially lethal with about 5-10% of adult escapers, clu^ΔW/clu^ΔW;park¹/park¹ mutants do not yield any viable adults. On the other hand the larval locomotor deficit of either of the single mutants is not significantly worsened in the double mutants.

(Wang et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

clu^ΔW/clu^d08713 is rescued by clu^UAS.Tag:MYC/Scer\GAL4^Mef2.PR

(Wang et al., 2016)

Comments

The abnormal mitochondrial morphology - clustered, rounded mitochondria - observed in muscles 6 and 7 of clu^ΔW/clu^d08713 mutant larvae can be rescued to wild-type appearance by Scer\GAL4^Mef2.PR-driven expression of clu^UAS.Tag:MYC (which on its own also induces defects in mitochondrial morphology).

(Wang et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

clu^ΔW

(Wang et al., 2016, Wang et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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