Dper\dsx BAC clone (r 1.3 scaffold 0:3349345..3409770 , clone address 31M5).
Dper\dsx+t31M5 is a suppressor of sterile phenotype of dsxΔ
Dper\dsx+t31M5 is a suppressor of abnormal sex-determination phenotype of dsxΔ
Dper\dsx+t31M5 is a suppressor of abnormal courtship behavior phenotype of dsxΔ
Dper\dsx+t31M5, Dper\fru+t36N18, dsxΔ/dsx1, fruP1.LexA/fruAJ96u3 has abnormal song phenotype
The intersex phenotype and sterility of dsxΔ/dsxΔ flies can be rescued by combination with Dper\dsx+t31M5 (the morphology of external genitalia and penis of the xenotransgenic rescue males is comparable to males rescued with a wild-type D. melanogaster dsx transgene, only minor defects in sex comb formation are observed).
Similarly, the xenotransgene also rescues the courtship behavior of dsxΔ mutants without imparting any of the unique species-specific courtship behavior but also without transferring the quantitative differences in aspects of the courtship behavior that are shared between the species (e.g. the time spent with wings extended or the angle of the wing relative to the body axis). The xenotransgenic rescue males also do not show species-specific song interconversion but sing like Drosophila melanogaster with alternating bouts of pulse and sine song.
dsx1 fruP1.LexA/dsxΔ fruAJ96u3 double mutants rescued with Dana\dsx+t21E22 and Dana\fru+t20H8 also sing like melanogaster with both pulse and sine song elements present, whereas mutants rescued with Dper\dsx+t31M5 and Dper\fru+t36N18 only sing normal-pulse song but not the sine song (but still do not display any evidence of species-specific song interconversion).