FlyBase Allele Report: Dmel\GATAe[1]

General Information

Symbol

Dmel\GATAe¹

Species

D. melanogaster

Name

FlyBase ID

FBal0322779

Feature type

allele

Associated gene

Dmel\GATAe

Associated Insertion(s)

TI{TI}GATAe¹

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Okumura et al., 2016)

Mutagen

ends-out gene targeting

(Okumura et al., 2016)

I-SceI

(Okumura et al., 2016)

FLPase

(Okumura et al., 2016)

Progenitor genotype

Associated Insertion(s)

TI{TI}GATAe¹

Cytology

Description

Replacement of almost all the coding region of GATAe with the w[+] gene.

(Okumura et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:16,016,249..16,019,683 [+]

Comment:

Most of the GATAe coding sequence has been replaced with the w gene by targeted homologous recombination.

(Okumura et al., 2016)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage | somatic clone

(Okumura et al., 2016)

lethal - all die before end of first instar larval stage (with Df(3R)sbd45)

(Okumura et al., 2016)

lethal - all die before end of first instar larval stage | recessive

(Okumura et al., 2016)

Phenotype Manifest In

adult posterior midgut epithelium | somatic clone

(Okumura et al., 2016)

enterocyte of posterior adult midgut epithelium | somatic clone

(Okumura et al., 2016)

enteroendocrine cell of posterior adult midgut epithelium | somatic clone

(Okumura et al., 2016)

intestinal stem cell of posterior adult midgut epithelium | somatic clone

(Okumura et al., 2016)

Detailed Description

Statement

Reference

GATAe¹ homozygotes die as first instar larvae and display morphological defects in the midgut. Combination with Df(3R)sbd45 is also lethal. GATAe¹ homozygote somatic MARCM clones induced in the adult posterior midgut fail to grow and lack well-developed brush-border, they do not contain any mitotic intestinal stem cells (ISCs) and majority of them lacks ISCs (marked by Dl expression) altogether. The failure of GATAe¹ clones to grow is not due to apoptosis. nub MARCM clones also show decrease in the frequency of differentiated enterocytes and enteroendocrine cells (marked by nub and pros protein expression, respectively).

(Okumura et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

GATAe¹ has abnormal size | somatic clone | adult stage phenotype, non-suppressible by N^ICN.UAS/Scer\GAL4^Act5C.PI

(Okumura et al., 2016)

GATAe¹ has abnormal size | somatic clone | adult stage phenotype, non-suppressible by Scer\GAL4^Act5C.PI/BacA\p35^UAS.cUa

(Okumura et al., 2016)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

GATAe¹ has adult posterior midgut epithelium | somatic clone phenotype, non-suppressible by N^ICN.UAS/Scer\GAL4^Act5C.PI

(Okumura et al., 2016)

GATAe¹ has adult posterior midgut epithelium | somatic clone phenotype, non-suppressible by Scer\GAL4^Act5C.PI/BacA\p35^UAS.cUa

(Okumura et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The small size of GATAe¹ homozygote MARCM clone in adult posterior midgut is not rescued by expression of N^ICN.Scer\UAS under the control of Scer\GAL4^Act5C.PI.

(Okumura et al., 2016)

Xenogenetic Interactions

Statement

Reference

The small clone size as well as the lack of differentiated enterocytes and enteroendocrine cells in GATAe¹ homozygote MARCM clone in adult posterior midgut is not rescued by expression of BacA\p35^Scer\UAS.cUa under the control of Scer\GAL4^Act5C.PI.

(Okumura et al., 2016)

Complementation and Rescue Data

Fails to complement

GATAe²

(Hernández de Madrid and Casanova, 2018)

Partially rescued by

GATAe¹ is partially rescued by Scer\GAL4^Act5C.PI/GATAe^UAS.Tag:FLAG

(Okumura et al., 2016)

Comments

Expression of GATAe^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^Act5C.PI in GATAe¹ homozygote MARCM clones in adult posterior midgut weakly rescues the growth inhibition of GATAe¹ clones at day 7 post-clone induction as well as the loss of intestinal stem cells, differentiated enterocytes and enteroendocrine cells in these clones.

(Okumura et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

GATAe¹

(Zhai et al., 2017, Okumura et al., 2016)

dGATAe¹

(Hernández de Madrid and Casanova, 2018)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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