Somatic clones expressing cicUAS.Tag:HA under the control of Scer\GAL4Tub.PU contain a similar overall number of cells and intestinal stem cells in adult flies as controls and maintain a wild-type ratio of stem cells/clone.
The regeneration-associated proliferation normally observed in adult midgut upon Pseudomonas entomophila enteric infection is inhibited in cicScer\UAS.T:Ivir\HA1 expressing cells: the growth of somatic intestinal stem cell flip-out clones expressing cicScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4esg-NP5130 in which all the progeny of the intestinal stem cells born during the regeneration period are visibly marked (using tub-Gal80[ts] to limit the time of expression/clone induction) is significantly reduced compared to controls.
Scer\GAL4Tub.PU/cicUAS.Tag:HA is a suppressor of increased cell number | recessive | somatic clone | adult stage phenotype of kis10D26
Scer\GAL4Tub.PU/cicUAS.Tag:HA is a suppressor of increased cell number | somatic clone | adult stage phenotype of Scer\GAL4Tub.PU, trrJF03242
Scer\GAL4Tub.PU/cicUAS.Tag:HA is a suppressor of adult midgut epithelium | somatic clone phenotype of kis10D26
Scer\GAL4Tub.PU/cicUAS.Tag:HA is a suppressor of intestinal stem cell | somatic clone | adult stage phenotype of kis10D26
Scer\GAL4Tub.PU/cicUAS.Tag:HA is a suppressor of adult midgut epithelium | somatic clone | adult stage phenotype of Scer\GAL4Tub.PU, trrJF03242
Scer\GAL4Tub.PU/cicUAS.Tag:HA is a suppressor of intestinal stem cell | somatic clone | adult stage phenotype of Scer\GAL4Tub.PU, trrJF03242
cicUAS.Tag:HA, Scer\GAL4NP5130 is a suppressor | partially of adult midgut | somatic clone phenotype of Ras85DV12.S35.UAS, Scer\GAL4NP5130
cicUAS.Tag:HA, Scer\GAL4NP5130 is a suppressor | partially of intestinal stem cell | somatic clone | adult stage phenotype of Ras85DV12.S35.UAS, Scer\GAL4NP5130
The overgrowth and increased mitotic rate of intestinal stem cell somatic flip-out clones expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4esg-NP5130 (using tub-Gal80[ts] to limit the time of expression/clone induction) in the adult midgut is suppressed by co-expression of cicScer\UAS.T:Ivir\HA1; the number of mitotic cells per adult midgut is even lower than in wild-type.