FB2024_03 , released June 25, 2024
Allele: Dmel\TmcGal4
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General Information
Symbol
Dmel\TmcGal4
Species
D. melanogaster
Name
FlyBase ID
FBal0321088
Feature type
allele
Associated gene
Dmel\Tmc
Associated Insertion(s)
TI{GAL4}TmcGal4
Carried in Construct
Key Links
Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Guo et al., 2016)
Mutagen
ends-out gene targeting
(Guo et al., 2016)
Progenitor genotype
Associated Insertion(s)
TI{GAL4}TmcGal4
Cytology
Description

Sequence encoding a GAL4 driver and a w+* marker are inserted into the Tmc locus near the translation initiation codon of Tmc isoform PD, resulting in about 7,000 bp of the Tmc gene being replaced by the reporter construct and more than 1,000 amino acids (about one-half of the coding sequence) of Tmc being deleted.

(Guo et al., 2016)
Allele components
Component
Use(s)
Inserted element
TI{GAL4}
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      TmcGal4/Tmc1 mutant adult females have a significant increase in preference for softer 100 mM sucrose-containing, but not plain, agarose for egg laying, compared to controls; TmcGal4 homozygous, but not heterozygous, adult females also have a significant increase in preference for softer 100 mM sucrose-containing agarose for egg laying, compared to controls; TmcGal4/Tmc1 and TmcGal4 homozygous adult females have no difference in preference for 100 mM sucrose-containing (rather than plain) agarose for egg laying, compared to controls.

      (Wu et al., 2019)

      TmcGal4 homozygotes as well as TmcGal4/Df(3L)BSC576 mutants display significant larval locomotion defects: reduced crawling speeds, increased head cast frequencies and enhanced backward locomotion.

      TmcGal4 homozygote mutants do not display any defects in either the dendrite morphology of class I dopaminergic neurons or in axon projections of the Tmc-positive neurons in the ventral nerve cord.

      (Guo et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Expression of Mmus\Tmc1Scer\UAS.cGa or Mmus\Tmc2Scer\UAS.cGa - individually as well as together - under the control of Scer\GAL4Tmc-Gal4 rescues (or partially rescues in the case of Mmus\Tmc2Scer\UAS.cGa single expression) the larval locomotion defects characteristic for TmcGal4 homozygotes (increased head curl frequency, tendency to backward locomotion).

      (Guo et al., 2016)
      Complementation and Rescue Data
      Rescued by

      TmcGal4 is rescued by TmcUAS.cGa

      (Guo et al., 2016)
      Comments

      Expression of TmcScer\UAS.cGa under the control of Scer\GAL4Tmc-Gal4 rescues the larval locomotion defects (reduced crawling speed, increased head cast frequency, tendency to crawl backward) characteristic for TmcGal4 homozygotes.

      (Guo et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      TmcGal4
      tmcGAL4
      (Li et al., 2022, Wu et al., 2019, Li et al., 2016)
      tmcGal4
      (Guo et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (4)