FB2024_03 , released June 25, 2024
Allele: Dmel\stac3B20
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General Information
Symbol
Dmel\stac3B20
Species
D. melanogaster
Name
FlyBase ID
FBal0320014
Feature type
allele
Associated gene
Dmel\stac
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Caviglia et al., 2016)
Mutagen
ethyl methanesulfonate
(Caviglia et al., 2016)
Progenitor genotype
Cytology
Description

T to A mutation in the 5' splice site of the last intron, resulting in intron retention and the introduction of a stop codon five nucleotides after the mutated splice site. This is predicted to result in a truncated protein lacking the C-terminal 30 amino acids of the wild-type product.

(Caviglia et al., 2016)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:25,064,118..25,064,118 [+]
Nucleotide change:

T25064118A

Reported nucleotide change:

T?A

Comment:

T to A mutation in the 5' splice site of the last intron, resulting in intron retention and the introduction of a stop codon five nucleotides after the mutated splice site. This is predicted to result in a truncated protein lacking the C-terminal 30 amino acids of the wild-type product.

(Caviglia et al., 2016)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      stac3B20/stac3B20 mutant embryos display interruptions in the dorsal trunk tracheal lumen, but not the tracheal epithelium, at points where tracheal tip cells normally meet to form anastomoses. The fusion cells are properly specified, and migrate, connect, polarize, and expand apical membrane domains and lumen, but fail to fuse their apical domains.

      The presence of a single stac3B20/stac3B20 fusion cell in stac3B20/+ embryos leads to a tracheal luminal fusion defect.

      stac3B20/Df(3R)BSC493 mutant embryos display defects in dorsal branch tube fusion.

      (Caviglia et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of dndScer\UAS.cKa, Rab7Scer\UAS.P\T.T:Avic\GFP-YFP or Rab39Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4dysf.C1.7 fails to rescue the tracheal fusion defects of stac3B20/stac3B20 mutant embryos.

      (Caviglia et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      stac3B20 is rescued by Scer\GAL4btl.PU/stacUAS.A.EGFP

      (Caviglia et al., 2016)
      Partially rescued by

      stac3B20 is partially rescued by Scer\GAL4dysf.C1.7/stacUAS.A.EGFP

      (Caviglia et al., 2016)

      stac3B20 is partially rescued by Scer\GAL4dysf.C1.7/stacUAS.B.EGFP

      (Caviglia et al., 2016)

      stac3B20 is partially rescued by stacTag:TY1,Tag:CS(TEVp),sfGFP,Tag:FLAG

      (Caviglia et al., 2016)
      Not rescued by

      stac3B20 is not rescued by Scer\GAL4dysf.C1.7/stac3B20.UAS.A.EGFP

      (Caviglia et al., 2016)
      Comments

      Expression of stacT:Scer\TY1,T:Zzzz\TEV.CS,T:Avic\GFP-SF,T:Zzzz\FLAG, or stacScer\UAS.B.T:Avic\GFP-EGFP under the control of Scer\GAL4dysf.C1.7, but not stac3B20.Scer\UAS.A.T:Avic\GFP-EGFP under the control of Scer\GAL4dysf.C1.7, in embryos partially rescues the tracheal defects of stac3B20/stac3B20 embryos.

      Expression of stacScer\UAS.A.T:Avic\GFP-EGFP under the control of Scer\GAL4dysf.C1.7 partially rescues, or under the control of Scer\GAL4btl.PU fully rescues the tracheal defects of stac3B20/stac3B20 embryos.

      (Caviglia et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      stac3B20
      (Tsarouhas et al., 2023, Camelo et al., 2022, Caviglia et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)