FlyBase Allele Report: Dmel\Ubr3[B]

General Information

Symbol

Dmel\Ubr3^B

Species

D. melanogaster

Name

FlyBase ID

FBal0319860

Feature type

allele

Associated gene

Dmel\Ubr3

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Graves et al., 2020, Li et al., 2016)

Progenitor genotype

Cytology

Description

Amino acid replacement: L788term.

(Graves et al., 2020, Li et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:7,947,596..7,947,596 [+]

Nucleotide change:

T7947596A

Amino acid change:

L788term | Ubr3-PB; L788term | Ubr3-PC; L788term | Ubr3-PA

Reported amino acid change:

L788term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Li et al., 2016)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal auditory perception | adult stage

(Li et al., 2016)

abnormal developmental rate | somatic clone

(Li et al., 2016)

abnormal neurophysiology | adult stage

(Li et al., 2016)

increased cell death | somatic clone

(Li et al., 2016)

lethal - all die before end of first instar larval stage

(Li et al., 2016)

Phenotype Manifest In

Johnston organ | P-stage | somatic clone

(Li et al., 2016)

photoreceptor neuron | somatic clone

(Li et al., 2016)

scolopidium | P-stage | somatic clone

(Li et al., 2016)

Detailed Description

Statement

Reference

Ubr3^B hemizygotes die as first instar larvae.

Ubr3^B/Ubr3^B mutant clones exhibit increased apoptosis, and show delayed differentiation of photoreceptors in the morphogenetic furrow of eye imaginal discs.

(Li et al., 2016)

Pupae with Ubr3^B mutant clones in the Johnston's organ exhibit scolopidia detached from the hinge of the second and third antennal segment and in adulthood display significant reduction in the sound evoked potentials in electroantennogram measurements.

(Li et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Ubr3^B has abnormal neuroanatomy | P-stage | somatic clone phenotype, enhanceable | somatic clone by Scer\GAL4^Act.PU/ck^UAS.GFP

(Li et al., 2016)

Suppressed by

Statement

Reference

Ubr3^B has abnormal developmental rate | somatic clone phenotype, suppressible by Scer\GAL4^Act.PU/cos^KK106270

(Li et al., 2016)

Ubr3^B has increased cell death | somatic clone phenotype, suppressible by Scer\GAL4^Act.PU/BacA\p35^UAS.cUa

(Li et al., 2016)

NOT suppressed by

Statement

Reference

Ubr3^B has abnormal developmental rate | somatic clone phenotype, non-suppressible by Scer\GAL4^Act.PU/BacA\p35^UAS.cUa

(Li et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

Ubr3^B has Johnston organ | P-stage | somatic clone phenotype, enhanceable | somatic clone by Scer\GAL4^Act.PU/ck^UAS.GFP

(Li et al., 2016)

Ubr3^B has scolopidium | P-stage | somatic clone phenotype, enhanceable | somatic clone by Scer\GAL4^Act.PU/ck^UAS.GFP

(Li et al., 2016)

Ubr3^B has Johnston organ | P-stage | somatic clone phenotype, enhanceable by Sans²⁴⁵/Sans[+]

(Li et al., 2016)

Ubr3^B has scolopidium | P-stage | somatic clone phenotype, enhanceable by Sans²⁴⁵/Sans[+]

(Li et al., 2016)

Ubr3^B has Johnston organ | P-stage | somatic clone phenotype, enhanceable by Cad99C^57A/Cad99C[+]

(Li et al., 2016)

Ubr3^B has scolopidium | P-stage | somatic clone phenotype, enhanceable by Cad99C^57A/Cad99C[+]

(Li et al., 2016)

Suppressed by

Statement

Reference

Ubr3^B has photoreceptor neuron | somatic clone phenotype, suppressible by Scer\GAL4^Act.PU/cos^KK106270

(Li et al., 2016)

NOT suppressed by

Statement

Reference

Ubr3^B has photoreceptor neuron | somatic clone phenotype, non-suppressible by Scer\GAL4^Act.PU/BacA\p35^UAS.cUa

(Li et al., 2016)

NOT Enhancer of

Statement

Reference

Ubr3^B/Ubr3^B is a non-enhancer | somatic clone of Johnston organ | P-stage | somatic clone phenotype of Scer\GAL4^Act.PU, ck^GD1408

(Li et al., 2016)

Ubr3^B/Ubr3^B is a non-enhancer | somatic clone of scolopidium | P-stage | somatic clone phenotype of Scer\GAL4^Act.PU, ck^GD1408

(Li et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of cos^KK106270 under the control of Scer\GAL4^Act.PU rescues the delayed photoreceptor differentiation phenotype of Ubr3^B/Ubr3^B mutant clones.

(Li et al., 2016)

The scolopidia detachment phenotype observed in Ubr3^B mutant clones in the pupal Johnston's organ is significantly enhanced by expression of ck^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^Act.PU in the mutant cells or by combination with a single copy of either Sans²⁴⁵ or Cad99C^57A.

Flies carrying Ubr3^B mutant clones expressing (under the Scer\GAL4^Act.PU driver) ck^GD1408 in the Johnston's organ display nearly complete detachment of scolopidia from the hinge of the second and third antennal segment - similar to animals with clones expressing the ck^GD1408 alone.

(Li et al., 2016)

Xenogenetic Interactions

Statement

Reference

Expression of BacA\p35^Scer\UAS.cUa under the control of Scer\GAL4^Act.PU suppresses the apoptosis phenotype, but fails to rescue the delayed photoreceptor differentiation phenotype of Ubr3^B/Ubr3^B mutant clones.

(Li et al., 2016)

Complementation and Rescue Data

Fails to complement

Ubr3^G0307a

(Li et al., 2016)

Rescued by

Ubr3^B is rescued by Ubr3^+t18.3

(Li et al., 2016)

Ubr3^B is rescued by Scer\GAL4^Act.PU/Ubr3^UAS.cLa

(Li et al., 2016)

Ubr3^B is rescued by Scer\GAL4^Act.PU/Ubr3^{UAS.cLa.Tag:FLAG}

(Li et al., 2016)

Not rescued by

Ubr3^B is not rescued by Scer\GAL4^Act.PU/Ubr3^{RINGmut.UAS.Tag:FLAG}

(Li et al., 2016)

Comments

Ubr3^B fails to complement the lethality of Ubr3^G0307a.

Ubr3^+t18.3 fully rescues the lethality of Ubr3^B hemizygous mutants.

(Li et al., 2016)

The sound transduction defects (reduced amplitude of sound evoked potentials) observed in adult flies carrying Ubr3^B mutant clones in the Johnston's organ can be rescued by expression of Ubr3^Scer\UAS.cLa driven by Scer\GAL4^Act.PU in the mutant cells.

The detached scolopidia phenotype observed in pupae bearing Ubr3^B mutant clones in the Johnston's organ can be rescued by expression of Ubr3[Scer\UAS.cLa. T:Zzzz \FLAG] (but not Ubr3^{RINGmut.Scer\UAS.T:Zzzz\FLAG}) driven by Scer\GAL4^Act.PU in the mutant cells.

(Li et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

67186

y¹ w^* Ubr3^B P{neoFRT}19A/FM7c, P{GAL4-Kr.C}DC1, P{UAS-GFP.S65T}DC5, sn⁺

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Ubr3^B

(Markus et al., 2023, Graves et al., 2020, Ray et al., 2019)

XHSY186

(Graves et al., 2020)

XM35B

(Graves et al., 2020)

ubr3^B

(Li et al., 2016, Li et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

Open Close