FB2024_03 , released June 25, 2024
Allele: Dmel\Arl2Δ156
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General Information
Symbol
Dmel\Arl2Δ156
Species
D. melanogaster
Name
FlyBase ID
FBal0318764
Feature type
allele
Associated gene
Dmel\Arl2
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Chen et al., 2016)
Progenitor genotype
P{XP}COX7Ad04921
(Chen et al., 2016)
Caused by aberration
Df(3R)Arl2Δ156
Cytology
Description

Imprecise excision of the progenitor insertion, resulting in deletion of the entire coding region of Arl2, deletion of the C-terminal coding region of CG9601 and disruption of the 5' UTR of COX7A.

(Chen et al., 2016)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3R:8,340,990..8,342,730 [+]
Comment:

A deletion resulting from the imprecise excision of the progenitor insertion which deletes sequences from -189 to +1551 relative to the Arl2 ATG start codon.

(Chen et al., 2016)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Arl2T30N.Scer\UAS driven by Scer\GAL4insc.PU results in a significant increase in neuroblast number in the third instar larval central brain; introduction of heterozygous Arl2Δ156/+ further enhances neuroblast overgrowth at 72hr (and even further at 84hr) after larval hatching at 29[o]C. Arl2Δ156/+ larvae that also have expression of Arl2T30N.Scer\UAS driven by Scer\GAL4insc.PU have a slightly extended larval stage and more than half of metaphase neuroblasts have shorter misoriented spindles (compared to wild type), with a small percentage dividing orthogonally. Unlike wild type telophase neuroblasts (where daughter cell sizes are distinct, with a NB/GMC diameter ratio of around 2), larvae with Arl2T30N.Scer\UAS overexpressed by Scer\GAL4insc.PU have a reduced ratio (around 1.65); introduction of Arl2Δ156/+ further reduces the ratio (around 1.5).

      Arl2Δ156/Arl2Δ156 clones have extra neuroblasts in both type I and II neuroblast lineages, compared to a single neuroblast in control clones (third instar larval central brain). Wild type interphase neuroblasts contain one major microtubule aster organized by the centrosome, whereas Arl2Δ156/Arl2Δ156 clones do not organize an aster and metaphase neuroblasts do not assemble mitotic spindle.

      (Chen et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of Arl2WT.Scer\UAS and COX7AScer\UAS.cCa driven by Scer\GAL4tub.PU rescues embryonic lethality in Arl2Δ156/Arl2Δ156 mutants.

      (Chen et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference

      Severe microtubule abnormalities and neuroblast overgrowth seen in Arl2Δ156/Arl2Δ156 mutant neuroblast clones is well rescued by expression of Mmus\Arl2Scer\UAS.cCa.

      (Chen et al., 2016)
      Complementation and Rescue Data
      Partially rescued by

      Arl2Δ156 is partially rescued by Scer\GAL4insc.PU/Arl2WT.UAS

      (Chen et al., 2016)
      Comments

      Expression of Arl2WT.Scer\UAS rescues the phenotype of extra neuroblasts in both type I and II neuroblast lineages seen in Arl2Δ156/Arl2Δ156 clones (Scer\GAL4insc.PU).

      (Chen et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Arl2Δ156
      arl2Δ156
      (Chen et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)