A Ubi-p63E promoter drives expression of a genomic fragment containing the 2.6kb CTCF open reading frame. The protein is tagged at the N-terminal end with "One-STrEP" (two copies of Tag:StrepII in tandem arrangement).
CTCFUbi-p63E.Tag:StrepII rescues CTCFP35.2/CTCFy+1
CTCFUbi-p63E.Tag:StrepII rescues CTCF9/CTCFy+1
CTCFUbi-p63E.Tag:StrepII rescues CTCFy+1
CTCFUbi-p63E.Tag:StrepII rescues CTCF9
CTCFUbi-p63E.T:Zzzz\StrepII rescues viability of CTCFy+1/CTCFP35.2, CTCFy+1/CTCF9, CTCFy+1/CTCFy+1 or CTCF9/CTCF9 flies.