FB2024_03 , released June 25, 2024
Allele: Dmel\Gr47aUAS.cLa
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General Information
Symbol
Dmel\Gr47aUAS.cLa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Lee
FlyBase ID
FBal0316431
Feature type
allele
Associated gene
Dmel\Gr47a
Associated Insertion(s)
Carried in Construct
P{UAS-Gr47a.L}
Key Links
Transgenic product class
cDNA
(Lee et al., 2015)
wild_type
(Lee et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Lee et al., 2015)
wild_type
(Lee et al., 2015)
Mutagen
in vitro construct
(Lee et al., 2015)
Progenitor genotype
Carried in construct
P{UAS-Gr47a.L}
Cytology
Description

UASt regulatory sequences drive expression of a full length Gr47a cDNA.

(Lee et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Gr47a
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      As in controls, expression of Gr47aScer\UAS.cLa under the control of Scer\GAL4Gr66a.PD does not elicit action potentials in sugar-responsive gustatory receptor neurons upon application of strychnine.

      (Lee et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      As in control flies, co-expression of Gr32aScer\UAS.cMa, Gr33aScer\UAS.cMa, Gr47aScer\UAS.cLa and Gr66aScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Gr5a.PUn does not elicit action potentials in sugar-responsive gustatory receptor neurons upon application of strychnine.

      (Lee et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Gr47aUAS.cLa/Scer\GAL4Gr36a.6 rescues Gr47a1

      (Lee et al., 2015)

      Gr47aUAS.cLa/Scer\GAL4Gr39b.4.4 rescues Gr47a1

      (Lee et al., 2015)

      Gr47aUAS.cLa/Scer\GAL4Gr8a.0.7 rescues Gr47a1

      (Lee et al., 2015)
      Fails to rescue

      Gr47aUAS.cLa/Scer\GAL4Gr47a.7.321 fails to rescue Gr47a1

      (Lee et al., 2015)

      Gr47aUAS.cLa/Scer\GAL4Gr59c.5.5 fails to rescue Gr47a1

      (Lee et al., 2015)

      Gr47aUAS.cLa/Scer\GAL4Gr93a.5 fails to rescue Gr47a1

      (Lee et al., 2015)

      Gr47aUAS.cLa/Scer\GAL4Gr98b.8.9 fails to rescue Gr47a1

      (Lee et al., 2015)
      Comments

      Expression of Gr47aScer\UAS.cLa under the control of Scer\GAL4Gr47a.7.321 does not rescue the impairment in avoiding strychnine seen in Gr47a1 mutant flies.

      Expression of Gr47aScer\UAS.cLa under the control of Scer\GAL4Gr36a.6, Scer\GAL4Gr39b.4.4 or Scer\GAL4Gr8a.0.7 rescues the impairment in strychnine avoidance seen in Gr47a1 mutant flies. No rescue is seen with Scer\GAL4Gr59c.5.5, Scer\GAL4Gr93a.5 or Scer\GAL4Gr98b.8.9. The loss of strychnine-induced action potentials in S6 is rescued when Gr47aScer\UAS.cLa is expressed under the control of Scer\GAL4Gr59c.5.5 or Scer\GAL4Gr98b.8.9, but not with Scer\GAL4Gr93a.5.

      Expression of Gr47aScer\UAS.cLa under the control of Scer\GAL4Gr36a.6 rescues the proboscis extension response defects seen in Gr47a1 mutant flies upon application of strychnine.

      (Lee et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Gr47aScer\UAS.cLa
      Gr47aUAS.cLa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Lee
      Secondary FlyBase IDs
        References (1)