FB2024_03 , released June 25, 2024
Allele: Hsap\RELAAD.R20A02
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General Information
Symbol
Hsap\RELAAD.R20A02
Species
H. sapiens
Name
FlyBase ID
FBal0304500
Feature type
allele
Associated gene
Hsap\RELA
Associated Insertion(s)
Carried in Construct
P{R20A02-p65.AD}
PBac{R20A02-p65.AD}
Also Known As
R20A02
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Aso et al., 2014)
Progenitor genotype
Carried in construct
P{R20A02-p65.AD}
PBac{R20A02-p65.AD}
Cytology
Description

The R20A02 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
GMR20A02
Encoded product / tool
p65(AD)::Zip+
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      larval mushroom body output neuron h1

      Comment: when combined with P{R28A10-GAL4.DBD} (combination referred to as 'SS00928')

      when combined with P{R28A10-GAL4.DBD} (combination referred to as 'SS01725')

      (Truman et al., 2023, Saumweber et al., 2018)
      larval mushroom body output neuron h2

      Comment: when combined with P{R28A10-GAL4.DBD} (combination referred to as 'SS00928')

      when combined with P{R28A10-GAL4.DBD} (combination referred to as 'SS01725')

      (Truman et al., 2023, Saumweber et al., 2018)
      adult stage
      mushroom body output neuron 8

      Comment: when combined with P{R94B10-GAL4.DBD} (combination referred to as 'MB110C')

      (Scaplen et al., 2021, Aso et al., 2014)
      mushroom body output neuron 8 | intense

      Comment: when combined with P{R94B10-GAL4.DBD} (combination referred to as 'MB110C')

      (Aso et al., 2014)
      mushroom body output neuron 9 | intense

      Comment: when combined with P{R94B10-GAL4.DBD} (combination referred to as 'MB110C')

      (Aso et al., 2014)
      adult brain | faint | restricted

      Comment: when combined with P{R11F03-GAL4.DBD} (combination referred to as 'SS02707')

      when combined with P{R93G02-GAL4.DBD} (combination referred to as 'SS02709')

      (Robie et al., 2017)
      adult brain | restricted

      Comment: when combined with P{R11F03-GAL4.DBD} (combination referred to as 'SS02707')

      when combined with P{R50H07-GAL4.DBD} (combination referred to as 'SS02708')

      (Robie et al., 2017)
      adult optic lobe neuron | subset

      Comment: when combined with P{R11F03-GAL4.DBD} (combination referred to as 'SS02707')

      when combined with P{R93G02-GAL4.DBD} (combination referred to as 'SS02709')

      (Robie et al., 2017)
      adult ellipsoid body ring neuron R1

      Comment: when combined with P{R11F03-GAL4.DBD} (combination referred to as 'SS02707')

      when combined with P{R93G02-GAL4.DBD} (combination referred to as 'SS02709')

      (Robie et al., 2017)
      adult ellipsoid body ring neuron R3

      Comment: when combined with P{R11F03-GAL4.DBD} (combination referred to as 'SS02707')

      when combined with P{R93G02-GAL4.DBD} (combination referred to as 'SS02709')

      (Robie et al., 2017)
      adult ellipsoid body ring neuron R4

      Comment: when combined with P{R11F03-GAL4.DBD} (combination referred to as 'SS02707')

      when combined with P{R93G02-GAL4.DBD} (combination referred to as 'SS02709')

      (Robie et al., 2017)
      adult optic lobe neuron | faint | subset

      Comment: when combined with P{R93G02-GAL4.DBD} (combination referred to as 'SS02709')

      (Robie et al., 2017)
      adult lateral horn PD2f1 neuron

      Comment: when combined with P{R67F03-GAL4.DBD} (combination referred to as 'LH2453')

      (Dolan et al., 2019)
      mushroom body output neuron 9

      Comment: when combined with P{R94B10-GAL4.DBD} (combination referred to as 'MB110C')

      (Scaplen et al., 2021)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Inhibition (using Hsap\KCNJ25XUAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R28A10 and Hsap\RELAAD.R20A02 in third instar larvae does not significantly affect short-term associative appetitive olfactory memory following training with fructose, innate odor (n-amylacetate) preference or innate fructose preference, compared to controls.

      (Saumweber et al., 2018)

      Blocking protein synthesis (using Rcom\RA[cs2.UAS] at 30[o]C) for the full 24 h between spaced training and testing in neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R94B10 and Hsap\RELAAD.R20A02, significantly impairs aversive olfactory long term memory in adults, compared to controls.

      Acute inhibition of endocytosis (using shi1.UAS at 32[o]C) in neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R94B10 and Hsap\RELAAD.R20A02 during testing, 24 h after spaced, but not massed, training significantly impairs aversive olfactory long term memory in adults, compared to controls.

      (Wu et al., 2017)

      Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R94B10 and Hsap\RELAAD.R20A02 in adult females in an area of an arena significantly increases attraction to that area, compared to controls.

      Thermogenetic activation (using TrpA1UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R94B10 and Hsap\RELAAD.R20A02 in adults significantly increases sleep, compared to controls.

      (Aso et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (11)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Split-GAL4 transgene, constructed as described in FBrf0212052.

      (Aso et al., 2014)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hsap\RELAAD.R20A02.T:Zzzz\Zip+
      Hsap\RELAAD.R20A02
      Name Synonyms
      Secondary FlyBase IDs
        References (22)