Expression of Dg^Scer\UAS.cYa in the muscle under the control of Scer\GAL4^Mef2.PU has no effect on viability.

Expression of Dg^Scer\UAS.cYa in the nervous system under the control of any of Scer\GAL4^elav.PU, Scer\GAL4^insc.PU or Scer\GAL4^wor.PU leads to high levels of embryonic lethality.

The brains of larvae expressing Dg^Scer\UAS.cYa under the control of Scer\GAL4^insc.PU contain dense cellular masses that overgrow the normal brain contour, protruding above the neural lamina. The majority of mutants die during the larval stages, but a few escapers make it to adulthood. Adult brains also contain lumps, but fewer than are seen in larvae. The lump-like structures are irregular in size and size correlate with level of Dg expression. They 'lumps' are not tumors: asymmetry of cell division and major differentiation processes appear unaffected. This phenotype can be partially rescued by nitric oxide treatment.

Brain clones expressing Dg^Scer\UAS.cYa under the control of Scer\GAL4^Act5C.PP do not show any obvious abnormalities at one day after induction, but after 3 days the cells start to aggregate and form three-dimensional masses that begin to separate from the rest of the brain tissue.

Expression of Dg^Scer\UAS.cYa in photoreceptor neurons under the control of either Scer\GAL4^GMR.PU or in mir-310 expressing neurons under the control of Scer\GAL4^NP4255 results in premature axon termination in the optic lobe.

Expression of Dg^Scer\UAS.cYa in mir-310 expressing neurons under the control of Scer\GAL4^NP4255 results in an increase in M-phase neuroblasts compared to controls.