Ubi-p63E promoter sequences drive expression of full-length rhea which carries the amino acid replacement R367A in the IBS-1 domain (this mutation is designed to disrupt the interaction of rhea with the integrin cytoplasmic tail) and the amino acid replacements L2103A and I2104A in the IBS-2 domain (these mutations should disrupt binding of the IBS-2 domain to the integrin cytoplasmic tail). The protein is tagged with EGFP.
rheaR367A.LI.AA.Ubi-p63E.EGFP fails to rescue Df(3L)79a
The germband retraction, dorsal closure and myotendinous junction defects seen in embryos lacking maternal and zygotic rhea function (homozygous Df(3L)79a embryos derived from homozygous Df(3L)79a female germline clones) are not rescued by expression of rheaR367A.LI.AA.Ubi-p63E.T:Avic\GFP-EGFP.