lethal (with Df(3R)Exel6198)
short lived (with Df(3R)Exel6198), with β-PheRSA158W
β-PheRS322/Df(3R)Exel6198 (β-PheRS null) flies rescued with the β-PheRSA158W mutant construct have no obvious phenotypes, showing normal developmental time, body size and bristle shape.
β-PheRS322/Df(3R)Exel6198 (β-PheRS null) flies rescued with the β-PheRSA158W mutant construct show normal locomotion (climbing) ability over several weeks. However, survival rates drop after 50 days, compared to controls.
α-PheRSA456G.UAS, β-PheRS+tLa, Df(3R)Exel6198/β-PheRS322, Scer\GAL4Act5C.PI has increased cell death phenotype
α-PheRSA456G.UAS, Df(3R)Exel6198/β-PheRS322, β-PheRSA158W, Scer\GAL4en-e16E has abnormal size phenotype
α-PheRSA456G.UAS, Df(3R)Exel6198/β-PheRS322, β-PheRSA158W, Scer\GAL4ppl.PP has lethal | nutrition conditional phenotype
α-PheRSA456G.UAS, Df(3R)Exel6198/β-PheRS322, β-PheRSA158W, Scer\GAL4Act5C.PI has lethal phenotype
α-PheRSA456G.UAS, β-PheRS+tLa, Df(3R)Exel6198/β-PheRS322, Scer\GAL4en-e16E has increased cell death phenotype
α-PheRSA456G.UAS, β-PheRS+tLa, Df(3R)Exel6198/β-PheRS322, Scer\GAL4en-e16E has decreased cell number phenotype
α-PheRSA456G.UAS, β-PheRS+tLa, Df(3R)Exel6198/β-PheRS322, Scer\GAL4en-e16E has abnormal size phenotype
α-PheRSA456G.UAS, β-PheRS+tLa, Df(3R)Exel6198/β-PheRS322, Scer\GAL4ppl.PP has lethal | nutrition conditional phenotype
α-PheRSA456G.UAS, Df(3R)Exel6198/β-PheRS322, β-PheRSA158W, Scer\GAL4ey.PH has ommatidium phenotype
α-PheRSA456G.UAS, β-PheRS+tLa, Df(3R)Exel6198/β-PheRS322, Scer\GAL4en-e16E has wing phenotype
α-PheRSA456G.UAS, β-PheRS+tLa, Df(3R)Exel6198/β-PheRS322, Scer\GAL4ey.PH has ommatidium phenotype
α-PheRSA456G.UAS, Df(3R)Exel6198/β-PheRS322, β-PheRSA158W, Scer\GAL4en-e16E has wing phenotype
In β-PheRS322/Df(3R)Exel6198 (β-PheRS null) flies rescued with β-PheRS+tLa, Scer\GAL4ey.PH-mediated expression of α-PheRSA456G.Scer\UAS results in eyes mostly displaying a regular surface, although a few ommatidia are not properly organized in certain regions. Some individual ones lack photoreceptors, causing ommatidia to be smaller and the retina to display a misaligned pattern.
In β-PheRS322/Df(3R)Exel6198 (β-PheRS null) flies rescued with β-PheRSA158W, Scer\GAL4ey.PH-mediated expression of α-PheRSA456G.Scer\UAS results in severe disruption of eye organization - most ommatidia are irregular, the entire eye is smaller and the eye surface is rough. The retina and ommatidia lack regular patterns, shapes and organization because of smaller ommatidia and absence of many cells.
Scer\GAL4Act5C.PI-mediated expression of α-PheRSA456G.Scer\UAS in adults (via use of Scer\GAL80ts.αTub84B) in a β-PheRS322/Df(3R)Exel6198 (β-PheRS null) background rescued with β-PheRS+tLa results in some elav-positive brain (neuronal) cells entering apoptosis. Many more cells enter apoptosis when this background is rescued with β-PheRSA158W.
In β-PheRS322/Df(3R)Exel6198 (β-PheRS null) flies rescued with β-PheRS+tLa or β-PheRSA158W, Scer\GAL4en-e16E-mediated expression of α-PheRSA456G.Scer\UAS results in decreased wing size , and in some wings entire regions of the posterior compartment are missing. In wing discs, the number of apoptotic cells is increased, while cell size is either similar or slightly bigger than controls, suggesting that cell number (proliferation) must be reduced.
In β-PheRS322/Df(3R)Exel6198 (β-PheRS null) flies rescued with β-PheRS+tLa or β-PheRSA158W, Scer\GAL4ppl.PP-mediated expression of α-PheRSA456G.Scer\UAS results in reduced pupariation rates when larvae are raised on Tyrosine-rich (but not alanine- or phenylalanine-rich or standard) food.
β-PheRS322 is rescued by β-PheRS+tLa
Df(3R)Exel6198/β-PheRS322 is rescued by β-PheRS+tLa
Df(3R)Exel6198/β-PheRS322 is rescued by β-PheRSTag:MYC
Df(3R)Exel6198/β-PheRS322 is rescued by β-PheRSA158W
β-PheRS+tLa rescues the lethality of β-PheRS322 and β-PheRS322/Df(3R)Exel6198.
β-PheRST:Hsap\MYC rescues the lethality of β-PheRS322/Df(3R)Exel6198.
β-PheRSA158W rescues the lethality of β-PheRS322/Df(3R)Exel6198.