FB2024_03 , released June 25, 2024
Allele: Dmel\htsΔ100.αTub84B
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General Information
Symbol
Dmel\htsΔ100.αTub84B
Species
D. melanogaster
Name
FlyBase ID
FBal0301360
Feature type
allele
Associated gene
Dmel\hts
Associated Insertion(s)
Carried in Construct
P{αTub84B-hts.Δ100}
Key Links
Transgenic product class
inframe_deletion
(Pokrywka et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Pokrywka et al., 2014)
Mutagen
in vitro construct
(Pokrywka et al., 2014)
Progenitor genotype
Carried in construct
P{αTub84B-hts.Δ100}
Cytology
Description

αTub84B promoter sequences drive expression of the hts cDNA sequence with the last 91 nucleotides removed.

(Pokrywka et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
αTub84B
Encoded product / tool
hts
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Wild-type flies carrying two copies of htsΔ100.αTub84B are completely fertile.

      Wild-type flies carrying htsΔ100.αTub84B exhibit normal arrangements of both anterior/lateral and sub-cortical microtubules.

      There is no evidence of defects in oocyte nuclear position in flies carrying htsΔ100.αTub84B.

      Young, 1-4 day old hts1/hts10089 mutant females expressing two copies of htsΔ100.αTub84B display a number of egg chambers that are able to complete oogenesis, with most of the resulting eggs (~60%) able to support embryogenesis to at least the larval stage. Ovaries from these females contain developing egg chambers of various stages, and also egg chambers that have arrested prior to vitellogenesis. All egg chambers in these flies contain ring canals, even those that have failed to mature. As these females get older, the aborted egg chambers accumulate in the ovaries, resulting in an overall failure of oogenesis.

      hts1/hts10089 mutant females expressing two copies of htsΔ100.αTub84B only rarely (~5%) contain ectopic actin aggregates.

      (Pokrywka et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      htsΔ100.αTub84B rescues hts10089/hts1

      (Pokrywka et al., 2014)
      Comments

      Young, 1-4 day old hts1/hts10089 mutant females expressing two copies of htsΔ100.αTub84B display a number of egg chambers that are able to complete oogenesis, with most of the resulting eggs (~60%) able to support embryogenesis to at least the larval stage. Ovaries from these females contain developing egg chambers of various stages, and also egg chambers that have arrested prior to vitellogenesis. All egg chambers in these flies contain ring canals, even those that have failed to mature. As these females get older, the aborted egg chambers accumulate in the ovaries, resulting in an overall failure of oogenesis.

      hts1/hts10089 mutant females expressing two copies of htsΔ100.αTub84B only rarely (~5%) contain ectopic actin aggregates.

      (Pokrywka et al., 2014)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      htsΔ100.αTub84B
      htsΔ100
      (Pokrywka et al., 2014)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)