FB2024_03 , released June 25, 2024
Allele: Dmel\Dh31UAS.cKa
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General Information
Symbol
Dmel\Dh31UAS.cKa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Kunst
FlyBase ID
FBal0301210
Feature type
allele
Associated gene
Dmel\Dh31
Associated Insertion(s)
Carried in Construct
P{UAS-Dh31.K}
Key Links
Transgenic product class
wild_type
(Kunst et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Kunst et al., 2014)
Mutagen
in vitro construct
(Kunst et al., 2014)
Progenitor genotype
Carried in construct
P{UAS-Dh31.K}
Cytology
Description

UAS regulatory sequences drive expression of Dh31.

(Kunst et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Dh31
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Dh31Scer\UAS.cKa under the control of Scer\GAL4nSyb.PU suppresses nighttime sleep specifically during the second half of the night. The decrease in sleep is accompanied by an increase in sleep fragmentation.

      Expression of Dh31Scer\UAS.cKa under the control of either Scer\GAL4GMR21E02, Scer\GAL4GMR21C09 or Scer\GAL4GMR20D02 has no significant effect on the amount of nighttime sleep.

      Expression of Dh31Scer\UAS.cKa under the control of Scer\GAL4GMR20A02 results in a significant decrease in nighttime sleep compared to controls, with the effect being strongest in the second half of the night. The reduction in sleep is accompanied by decreased sleep consolidation and increase waking locomotor activity.

      Expression of Dh31Scer\UAS.cKa under the control of Scer\GAL4GMR43D05 significantly suppresses nighttime sleep. This phenotype is strongly reduced if the flies are also carrying Scer\GAL80cry.PS.

      (Kunst et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4GMR18H11/Dh31UAS.cKa rescues Dh31KG09001

      (Kunst et al., 2014)

      Dh31UAS.cKa/Scer\GAL4GMR20A02 rescues Dh31KG09001

      (Kunst et al., 2014)
      Comments

      Expression of Dh31Scer\UAS.cKa under the control of either Scer\GAL4GMR18H11 or Scer\GAL4GMR20A02 rescues waking during the 3 hours before dawn in homozygous Dh31KG09001 flies.

      (Kunst et al., 2014)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Dh31Scer\UAS.cKa
      Dh31UAS.cKa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Kunst
      Secondary FlyBase IDs
        References (1)