FB2024_03 , released June 25, 2024
Allele: Dmel\sqhEE.EGFP
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General Information
Symbol
Dmel\sqhEE.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0298058
Feature type
allele
Associated gene
Dmel\sqh
Associated Insertion(s)
Carried in Construct
P{sqh.EGFP.EE}
Key Links
Transgenic product class
missense_variant
(Vasquez et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Vasquez et al., 2014)
Mutagen
in vitro construct
(Vasquez et al., 2014)
Progenitor genotype
Carried in construct
P{sqh.EGFP.EE}
Cytology
Description

A genomic fragment containing the sqh gene locus, including 5' promoter and 3' termination sequences tagged at the C-terminal end with EGFP. The threonine at residue 20 and serine at residue 21 have both been replaced by alanine.

(Vasquez et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
sqh
Encoded product / tool
sqh
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Embryos expressing sqhEE.T:Avic\GFP-EGFP do not exhibit gastrulation defects.

      (Vasquez et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Other
      Statement
      Reference

      Rok1, sqhEE.EGFP has abnormal cell migration phenotype

      (Vasquez et al., 2014)
      Phenotype Manifest In
      Other
      Statement
      Reference

      Rok1, sqhEE.EGFP has embryo | gastrula stage phenotype

      (Vasquez et al., 2014)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Embryos expressing sqhEE.T:Avic\GFP-EGFP in a Rok1 mutant background fail to undergo mesoderm invagination.

      (Vasquez et al., 2014)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      sqhEE.EGFP fails to rescue sqh1

      (Vasquez et al., 2014)
      Comments

      Expression of sqhEE.T:Avic\GFP-EGFP does not rescue the nuclear migration and uneven cellularization seen in sqh1 mutant embryos. No adult flies are observed.

      (Vasquez et al., 2014)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      sqhEE.EGFP
      sqhEE.T:Avic\GFP-EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)