FlyBase Allele Report: Dmel\Or83c[UAS.cRa]

General Information

Symbol

Dmel\Or83c^UAS.cRa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Ronderos

FlyBase ID

FBal0297288

Feature type

allele

Associated gene

Dmel\Or83c

Associated Insertion(s)

Carried in Construct

P{UAS-Or83c.R}

Also Known As

UAS-Or83c

Key Links

Transgenic product class

wild_type

(Ronderos et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Ronderos et al., 2014)

Mutagen

in vitro construct

(Ronderos et al., 2014)

Progenitor genotype

Carried in construct

P{UAS-Or83c.R}

Cytology

Description

UAS regulatory sequences drive expression of Or83c.

(Ronderos et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Or83c

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology, with Or83c^MB11142, Scer\GAL4^Or23a.PC

(Ronderos et al., 2014)

abnormal neurophysiology, with Scer\GAL4^Or67d-GAL4-2

(Ha et al., 2014)

abnormal smell perception, with Or83c^MB11142, Scer\GAL4^Or23a.PC

(Ronderos et al., 2014)

abnormal smell perception, with Scer\GAL4^Or67d-GAL4-2

(Ha et al., 2014)

Phenotype Manifest In

antennal intermediate sensillum ai2, with Or83c^MB11142, Scer\GAL4^Or23a.PC

(Ronderos et al., 2014)

Detailed Description

Statement

Reference

Or83c^Scer\UAS.cRa mis-expressed under the control of Scer\GAL4^Or67d-GAL4-2 instead of the endogenous Or67d receptor gene respond to the Or83c-ligand farnesol.

(Ha et al., 2014)

Driving Or83c^Scer\UAS.cRa with Scer\GAL4^Or23a.PC in Or83c^MB11142 mutant background confers farnesol sensitivity to ai2b neurons.

(Ronderos et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Or83c^UAS.cRa, Scer\GAL4^Or67d-GAL4-2 has abnormal neurophysiology phenotype, enhanceable by ATP8B^f05203

(Ha et al., 2014)

Or83c^UAS.cRa, Scer\GAL4^Or67d-GAL4-2 has abnormal smell perception phenotype, enhanceable by ATP8B^f05203

(Ha et al., 2014)

Suppressor of

Statement

Reference

Scer\GAL4^{Orco.2.642.Hsim\VP22}/Or83c^UAS.cRa is a suppressor of abnormal smell perception phenotype of Orco²

(Ronderos et al., 2014)

Scer\GAL4^{Orco.2.642.Hsim\VP22}/Or83c^UAS.cRa is a suppressor of abnormal neurophysiology phenotype of Orco²

(Ronderos et al., 2014)

Scer\GAL4^Snmp1.PR/Or83c^UAS.cRa is a suppressor of abnormal smell perception phenotype of Snmp1^Z0429

(Ronderos et al., 2014)

Scer\GAL4^Snmp1.PR/Or83c^UAS.cRa is a suppressor of abnormal neurophysiology phenotype of Snmp1^Z0429

(Ronderos et al., 2014)

Other

Statement

Reference

Or67d^GAL4-2, Or83c^UAS.cRa, Scer\GAL4^Or67d-GAL4-2 has abnormal neurophysiology phenotype

(Ronderos et al., 2014)

Or67d^GAL4-2, Or83c^UAS.cRa, Scer\GAL4^Or67d-GAL4-2 has abnormal smell perception phenotype

(Ronderos et al., 2014)

Df(2L)dp-79b, Dp(2;2)dpp^d21, Or83c^UAS.cRa, Scer\GAL4^Or22a.8197 has abnormal smell perception phenotype

(Ronderos et al., 2014)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^{Orco.2.642.Hsim\VP22}/Or83c^UAS.cRa is a suppressor of antennal intermediate sensillum ai2 phenotype of Orco²

(Ronderos et al., 2014)

Scer\GAL4^Snmp1.PR/Or83c^UAS.cRa is a suppressor of antennal intermediate sensillum ai2 phenotype of Snmp1^Z0429

(Ronderos et al., 2014)

Other

Statement

Reference

Or67d^GAL4-2, Or83c^UAS.cRa, Scer\GAL4^Or67d-GAL4-2 has antennal trichoid sensillum at1 phenotype

(Ronderos et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Responses to farnesol are moderately reduced in Scer\GAL4^Or67d-GAL4-2>Or83c^Scer\UAS.cRa transgenic flies in an ATP8B^f05203 mutant background compared with transgenic expression in a wild-type genetic background.

(Ha et al., 2014)

Flies homozygous for Or67d^GAL4-2 and expressing Scer\GAL4^Or67d-GAL4-2>Or83c^Scer\UAS.cRa show restored spontaneous activity in at1 neurons, but to a distinct higher average firing rate. The mutant neurons expressing Or83c^Scer\UAS.cRa remain insensitive to the male-specific pheromone 11-cis-vaccenyl acetate (cVA), similar to Or67d^GAL4-2 mutants.

A single odorant out a panel of approximately 150 tested volatile compounds, farnesol elicited robust activity in the mutant at1 neurons expressing Scer\GAL4^Or67d-GAL4-2>Or83c^Scer\UAS.cRa. Importantly, farnesol does not activate wild-type at1 neurons.

Driving Or83c^Scer\UAS.cRa with Scer\GAL4^{Orco.2.642.T:Hsim\VP22} restores farnesol sensitivity to ai2b neurons in Orco² mutants.

Driving Or83c^Scer\UAS.cRa with Scer\GAL4^Snmp1.PR restores farnesol sensitivity to ai2b neurons in Snmp1^Z0429 mutants.

Or83c^Scer\UAS.cRa fails to confer farnesol sensitivity when mis-expressed under the control of Scer\GAL4^Or22a.8197 in a basiconic neuron context in a Df(2L)dp-79b/Dp(2;2)dpp^d21 ("Δhalo") genetic background.

(Ronderos et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^Or83c.PC/Or83c^UAS.cRa rescues Or83c^MB11142

(Ronderos et al., 2014)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Or83c^Scer\UAS.cRa

Or83c^UAS.cRa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Ronderos

Secondary FlyBase IDs

References (3)

Report Sections

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