UAS regulatory sequences drive expression of a full-length foxo open reading frame (ORF) that is tagged at the C-terminal end with three copies of the Tag:HA epitope. The ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or tag sequence.
visible | adult stage, with Scer\GAL4ey.PH
eye, with Scer\GAL4ey.PH
The expression of foxoUAS.ORF.GW.Tag:HA under the control of Scer\GAL4ey.PH induces a small eye phenotype; eye discs are small and show only a few columns of photoreceptor clusters.
The expression of foxoUAS.ORF.GW.Tag:HA under the control of Scer\GAL4AB1 induces a small third instar larval salivary gland, which presents more but smaller cells, with decreased cell growth (decreased endoreplication).
Expression of foxoScer\UAS.ORF.GW.T:Ivir\HA1 under the control of Scer\GAL4Bx-MS1096 results in male flies with severe wing size and patterning defects.
Expression of foxoScer\UAS.ORF.GW.T:Ivir\HA1 under the control of Scer\GAL4Bx-MS1096 results in female flies with weak wing size and patterning defects.
Scer\GAL4ey.PH, foxoUAS.ORF.GW.Tag:HA has decreased size | adult stage phenotype, enhanceable by TctpRNAi.UAS/Scer\GAL4ey.PH
Scer\GAL4ey.PH, foxoUAS.ORF.GW.Tag:HA has visible | adult stage phenotype, enhanceable by TctpRNAi.UAS/Scer\GAL4ey.PH
Scer\GAL4ey.PH, foxoUAS.ORF.GW.Tag:HA has decreased size | larval stage phenotype, suppressible | partially by TctpUAS.cHa/Scer\GAL4ey.PH
Scer\GAL4ey.PH, foxoUAS.ORF.GW.Tag:HA has decreased size | adult stage phenotype, suppressible | partially by TctpUAS.cHa/Scer\GAL4ey.PH
Scer\GAL4ey.PH, foxoUAS.ORF.GW.Tag:HA has visible | adult stage phenotype, suppressible | partially by TctpUAS.cHa/Scer\GAL4ey.PH
Scer\GAL4AB1, foxoUAS.ORF.GW.Tag:HA has decreased size | third instar larval stage phenotype, non-suppressible by 14-3-3εUAS.Tag:MYC, Scer\GAL4AB1
Scer\GAL4AB1, foxoUAS.ORF.GW.Tag:HA has decreased cell size | third instar larval stage phenotype, non-suppressible by 14-3-3εUAS.Tag:MYC, Scer\GAL4AB1
Scer\GAL4AB1, foxoUAS.ORF.GW.Tag:HA has decreased cell size | third instar larval stage phenotype, non-suppressible by Hsap\TPT1UAS.cHa, Scer\GAL4AB1
Scer\GAL4AB1, foxoUAS.ORF.GW.Tag:HA has decreased size | third instar larval stage phenotype, non-suppressible by Hsap\TPT1UAS.cHa, Scer\GAL4AB1
foxoUAS.ORF.GW.Tag:HA/Scer\GAL4ey.PH is an enhancer of decreased size | adult stage phenotype of Scer\GAL4ey.PH, TctpRNAi.UAS
foxoUAS.ORF.GW.Tag:HA/Scer\GAL4ey.PH is an enhancer of visible | adult stage phenotype of Scer\GAL4ey.PH, TctpRNAi.UAS
Scer\GAL4ey.PH, foxoUAS.ORF.GW.Tag:HA has eye phenotype, enhanceable by TctpRNAi.UAS/Scer\GAL4ey.PH
Scer\GAL4ey.PH, foxoUAS.ORF.GW.Tag:HA has eye phenotype, suppressible | partially by TctpUAS.cHa/Scer\GAL4ey.PH
Scer\GAL4ey.PH, foxoUAS.ORF.GW.Tag:HA has eye disc posterior to the morphogenetic furrow phenotype, suppressible | partially by TctpUAS.cHa/Scer\GAL4ey.PH
Scer\GAL4AB1, foxoUAS.ORF.GW.Tag:HA has embryonic/larval salivary gland | third instar larval stage phenotype, non-suppressible by 14-3-3εUAS.Tag:MYC, Scer\GAL4AB1
Scer\GAL4AB1, foxoUAS.ORF.GW.Tag:HA has embryonic/larval salivary gland | third instar larval stage phenotype, non-suppressible by Hsap\TPT1UAS.cHa, Scer\GAL4AB1
foxoUAS.ORF.GW.Tag:HA/Scer\GAL4ey.PH is an enhancer of eye phenotype of Scer\GAL4ey.PH, TctpRNAi.UAS