FlyBase Allele Report: Scer\GAL4[VT039046]

General Information

Symbol

Scer\GAL4^VT039046

Species

S. cerevisiae

Name

FlyBase ID

FBal0296104

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{VT039046-GAL4}

Also Known As

Dh44-GAL4, VT039046

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Meadows, 2015.3.13)

Progenitor genotype

Carried in construct

P{VT039046-GAL4}

Cytology

Description

A 2178 base pair fragment from the flanking non-coding or intronic region of Dh44 is fused upstream of a Drosophila synthetic core promoter (DSCP) followed by sequence encoding a GAL4 driver.

(Meadows, 2015.3.13)

Allele components

Component

Use(s)

Regulatory region(s)

VT039046

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

Additional Information

Statement

Reference

Axons from ScerGAL4^VT039046 expressing neurons terminate in the adult subesophageal zone with their dendritic compartments located near the pars intercerebralis and esophagus. Positive GRASP (GFP reconstitution across synaptic partners) signals between adult Hugin neurons labelled with EcollexA^GMR61H10 and DH44 neurons labelled with ScerGAL4^VT039046 were observed in the adult subesophageal zone, near the esophagus, and along the midline of the brain.

(King et al., 2017)

ScerGAL4^VT039046 drives expression in a subset of six ScerGAL4^Kurs58 positive neurons of the adult pars intercerebralis (PI) recapitulating endogenous Dh44 protein expression. GFP reconstitution across synaptic partners (GRASP) shows a connection between ScerGAL4^VT039046 positive PI neurons and EcollexA^{Clk.4.1M.T:HsimVP16} positive DN1p neurons.

(Cavanaugh et al., 2014)

Marker for

Reflects expression of

Dh44

(Barber et al., 2021, King et al., 2017, Cavanaugh et al., 2014)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor rhythm, with rpr^UAS.C

(Cavanaugh et al., 2014)

abnormal locomotor rhythm | adult stage | heat sensitive, with TrpA1^UAS.(B).cKa

(Cavey et al., 2016)

viable, with rpr^UAS.C

(Cavanaugh et al., 2014)

Phenotype Manifest In

Detailed Description

Statement

Reference

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28[o]C) of Scer\GAL4^VT039046 neurons in adults results in a significant reduction in the strength of circadian locomotor rhythms, compared to controls.

(Cavey et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^VT039046

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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