Expression of Scer\GAL4GMR.PF>Hsap\MAPT4xA.Scer\UAS.2N4R results in mild disruption of ommatidia.
Expression of Hsap\MAPT4xA.Scer\UAS.2N4R in the notal bristles of adult flies, under the control of Scer\GAL4Eq1 results in bristle loss.
Hsap\MAPT4xA.UAS.2N4R, Scer\GAL4GMR.PF has visible phenotype, enhanceable by Hsap\GSK3BUAS.cPa, Scer\GAL4GMR.PF
Hsap\MAPT4xA.UAS.2N4R, Scer\GAL4GMR.PF has ommatidium phenotype, enhanceable by Hsap\GSK3BUAS.cPa, Scer\GAL4GMR.PF
Hsap\MAPT4xA.UAS.2N4R, Scer\GAL4GMR.PF has ommatidium phenotype, suppressible by AmphA.UAS, Scer\GAL4GMR.PF
Hsap\MAPT4xA.UAS.2N4R, Scer\GAL4Eq has notal microchaeta phenotype, suppressible by Amph[+]/Amph5E3
Hsap\MAPT4xA.UAS.2N4R, Scer\GAL4GMR.PF has eye phenotype, suppressible by Amph[+]/Amph5E3
Hsap\MAPT4xA.UAS.2N4R, Scer\GAL4GMR.PF has ommatidium phenotype, suppressible by Amph[+]/Amph5E3
Hsap\MAPT4xA.UAS.2N4R, Scer\GAL4Eq has notal microchaeta phenotype, suppressible by AmphJF02883, Scer\GAL4Eq
Hsap\MAPT4xA.UAS.2N4R, Scer\GAL4GMR.PF has eye phenotype, suppressible by AmphJF02883, Scer\GAL4GMR.PF
Hsap\MAPT4xA.UAS.2N4R, Scer\GAL4GMR.PF has ommatidium phenotype, suppressible by AmphJF02883, Scer\GAL4GMR.PF
Hsap\MAPT4xA.UAS.2N4R, Scer\GAL4GMR.PF has eye phenotype, non-suppressible by AmphA.UAS, Scer\GAL4GMR.PF
Co-expression of Hsap\GSK3BScer\UAS.cPa increases Scer\GAL4GMR.PF>Hsap\MAPT4xA.Scer\UAS.2N4R-induced eye degeneration. The eyes have a greater degree of external disorganisation and extensive patches of depigmentation indicative of increased toxicity.
Eye-specific overexpression of AmphA.Scer\UAS (under the control of Scer\GAL4GMR.PF) does not significantly alter the size of Hsap\MAPT4xA.Scer\UAS.2N4R-induced rough eye, although the number of ommatidia is significantly reduced due to increased ommatidial fusions.
Knockdown of Amph, through expression of AmphJF02883 in the eye (under the control of Scer\GAL4GMR.PF) suppressed Hsap\MAPT4xA.Scer\UAS.2N4R-induced toxicity, leading to a 30% increase in eye surface, compared with flies expressing solely Hsap\MAPT4xA.Scer\UAS.2N4R.
Knockdown of Amph, through expression of AmphJF02883 in the notal bristles (under the control of Scer\GAL4Eq1)suppresses the Scer\GAL4Eq1>Hsap\MAPT4xA.Scer\UAS.2N4R-induced a bristle loss phenotype, resulting in 37% more notal bristles compared with flies expressing Scer\GAL4Eq1>Hsap\MAPT4xA.Scer\UAS.2N4R alone.
A Amph5E3 heterozygous background suppresses Hsap\MAPT4xA.Scer\UAS.2N4R-induced toxicity, leading to a 30% increase in eye surface, compared with flies expressing solely Hsap\MAPT4xA.Scer\UAS.2N4R.
A Amph5E3 heterozygous background suppresses the Scer\GAL4Eq1>Hsap\MAPT4xA.Scer\UAS.2N4R-induced a bristle loss phenotype, resulting in 37% more notal bristles compared with flies expressing Scer\GAL4Eq1>Hsap\MAPT4xA.Scer\UAS.2N4R alone.