FlyBase Allele Report: Dmel\hoip[1]

General Information

Symbol

Dmel\hoip¹

Species

D. melanogaster

Name

FlyBase ID

FBal0291685

Feature type

allele

Associated gene

Dmel\hoip

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Johnson et al., 2013)

Progenitor genotype

Cytology

Description

Amino acid replacement: G37E.

(Johnson et al., 2013)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:9,700,106..9,700,106 [+]

Nucleotide change:

G9700106A

Reported nucleotide change:

G?A

Amino acid change:

G37E | hoip-PA; G37E | hoip-PB

Reported amino acid change:

G37E

(Johnson et al., 2013)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape | embryonic stage

(Williams et al., 2015)

lethal - all die during embryonic stage

(Johnson et al., 2013)

lethal - all die during embryonic stage (with Df(2L)ED690)

(Johnson et al., 2013)

lethal - all die during embryonic stage (with hoip^k07104)

(Johnson et al., 2013)

Phenotype Manifest In

embryonic myoblast

(Williams et al., 2015)

embryonic somatic muscle cell

(Johnson et al., 2013)

embryonic somatic muscle cell (with Df(2L)ED690)

(Johnson et al., 2013)

embryonic somatic muscle cell (with hoip^k07104)

(Johnson et al., 2013)

muscle cell of dorsal oblique muscle 2 | embryonic stage

(Williams et al., 2015)

muscle cell of dorsal oblique muscle 3 | embryonic stage

(Williams et al., 2015)

muscle cell of dorsal oblique muscle 4 | embryonic stage

(Williams et al., 2015)

muscle cell of dorsal oblique muscle 5 | embryonic stage

(Williams et al., 2015)

muscle cell of lateral longitudinal muscle 1 | embryonic stage

(Williams et al., 2015)

muscle cell of lateral oblique muscle 1 | embryonic stage

(Williams et al., 2015)

muscle cell of lateral transverse muscle 1 | embryonic stage

(Williams et al., 2015)

muscle cell of lateral transverse muscle 2 | embryonic stage

(Williams et al., 2015)

muscle cell of lateral transverse muscle 3 | embryonic stage

(Williams et al., 2015)

muscle cell of lateral transverse muscle 4 | embryonic stage

(Williams et al., 2015)

myotube | embryonic stage

(Williams et al., 2015)

sarcomere | embryonic stage

(Williams et al., 2015)

Detailed Description

Statement

Reference

hoip¹ mutant embryos display defective myotube elongation of a subset of somatic myotubes, including the lateral longitudinal (LL1), dorsal oblique (DO3-5), lateral transverse (LT1-4) and lateral oblique (LO1); the dorsal oblique muscle 2 is decreased in size, despite elongating normally; these embryos also display defects in myoblast fusion and sarcomere assembly.

(Williams et al., 2015)

Somatic muscle morphology is aberrant in homozygous hoip¹ mutant embryos compared to heterozygous controls. In particular, the lateral transverse (LT1-4), lateral longitudinal (LL1), lateral oblique (LO1) and dorsal oblique (DO3-5) remain rounded rather than showing pronounced membrane extensions towards target sites as in wild type. Embryos fail to emerge from the chorion after embryogenesis. No PNS defects are detected.

Somatic muscle morphology is aberrant in hoip¹/Df(2L)ED690 mutant embryos compared to wild type. In particular, the lateral transverse (LT1-4), lateral longitudinal (LL1), lateral oblique (LO1) and dorsal oblique (DO3-5) remain rounded rather than showing pronounced membrane extensions towards target sites as in wild type. Embryos fail to emerge from the chorion after embryogenesis.

Somatic muscle morphology is aberrant in hoip¹/hoip^k07104 mutant embryos compared to wild type. In particular, the lateral transverse (LT1-4), lateral longitudinal (LL1), lateral oblique (LO1) and dorsal oblique (DO3-5) remain rounded rather than showing pronounced membrane extensions towards target sites as in wild type. Embryos fail to emerge from the chorion after embryogenesis. No PNS defects are detected.

Unlike in wild type nascent myotubes fail to elongate in hoip¹ mutant embryos after 30 minutes, even though the myotubes show an initial polarity. Wild type myotubes elongate to 50% of segment width after 30 minutes but hoip¹ mutant embryos even after 60 minutes. By this stage they have lost their polarity. hoip¹ mutants do not show any defects in attachment site recognition; as in wild type they extend filopodia exclusively in the direction of myotube polarity. Founder cell specification and the first round of myoblast fusion proceed normally in homozygous hoip¹ mutant embryos but the later rounds of fusion do not occur. However, the lack of fusion and elongation defects do not always correlate; DO1 and DA1 muscles elongate normally in hoip¹ embryos but have fewer nuclei, whereas LL1 and LO5 are multinucleate but fail to elongate.

(Johnson et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

hoip¹ has abnormal cell shape | embryonic stage phenotype, suppressible | partially by Tm2^1.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4}

(Williams et al., 2015)

hoip¹ has abnormal cell shape | embryonic stage phenotype, suppressible | partially by Tm2^2.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4}

(Williams et al., 2015)

hoip¹ has abnormal cell shape | embryonic stage phenotype, suppressible | partially by Tm2^3.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4}

(Williams et al., 2015)

hoip¹ has abnormal cell shape | embryonic stage phenotype, suppressible | partially by Scer\GAL4^{kirre-rP298-G4}/Tm2^{cDNA.UAS.EGFP}

(Williams et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

hoip¹ has myotube | embryonic stage phenotype, suppressible | partially by Tm2^2.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4}

(Williams et al., 2015)

hoip¹ has muscle cell of lateral longitudinal muscle 1 | embryonic stage phenotype, suppressible | partially by Tm2^2.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4}

(Williams et al., 2015)

hoip¹ has muscle cell of lateral transverse muscle 1 | embryonic stage phenotype, suppressible | partially by Tm2^2.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4}

(Williams et al., 2015)

hoip¹ has muscle cell of lateral transverse muscle 2 | embryonic stage phenotype, suppressible | partially by Tm2^2.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4}

(Williams et al., 2015)

hoip¹ has muscle cell of lateral transverse muscle 3 | embryonic stage phenotype, suppressible | partially by Tm2^2.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4}

(Williams et al., 2015)

hoip¹ has myotube | embryonic stage phenotype, suppressible | partially by Tm2^3.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4}

(Williams et al., 2015)

hoip¹ has muscle cell of lateral longitudinal muscle 1 | embryonic stage phenotype, suppressible | partially by Tm2^3.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4}

(Williams et al., 2015)

hoip¹ has muscle cell of dorsal oblique muscle 2 | embryonic stage phenotype, suppressible | partially by Tm2^3.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4}

(Williams et al., 2015)

hoip¹ has embryonic myoblast phenotype, suppressible | partially by Tm2^3.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4}

(Williams et al., 2015)

hoip¹ has sarcomere | embryonic stage phenotype, suppressible by Tm2^3.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4}

(Williams et al., 2015)

hoip¹ has myotube | embryonic stage phenotype, suppressible | partially by Scer\GAL4^{kirre-rP298-G4}/Tm2^{cDNA.UAS.EGFP}

(Williams et al., 2015)

hoip¹ has muscle cell of lateral longitudinal muscle 1 | embryonic stage phenotype, suppressible | partially by Scer\GAL4^{kirre-rP298-G4}/Tm2^{cDNA.UAS.EGFP}

(Williams et al., 2015)

hoip¹ has muscle cell of lateral transverse muscle 1 | embryonic stage phenotype, suppressible | partially by Scer\GAL4^{kirre-rP298-G4}/Tm2^{cDNA.UAS.EGFP}

(Williams et al., 2015)

hoip¹ has muscle cell of lateral transverse muscle 2 | embryonic stage phenotype, suppressible | partially by Scer\GAL4^{kirre-rP298-G4}/Tm2^{cDNA.UAS.EGFP}

(Williams et al., 2015)

hoip¹ has muscle cell of lateral transverse muscle 3 | embryonic stage phenotype, suppressible | partially by Scer\GAL4^{kirre-rP298-G4}/Tm2^{cDNA.UAS.EGFP}

(Williams et al., 2015)

hoip¹ has myotube | embryonic stage phenotype, suppressible | partially by Tm2^1.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4}

(Williams et al., 2015)

hoip¹ has muscle cell of lateral longitudinal muscle 1 | embryonic stage phenotype, suppressible | partially by Tm2^1.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4}

(Williams et al., 2015)

hoip¹ has muscle cell of lateral transverse muscle 1 | embryonic stage phenotype, suppressible | partially by Tm2^1.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4}

(Williams et al., 2015)

hoip¹ has muscle cell of lateral transverse muscle 2 | embryonic stage phenotype, suppressible | partially by Tm2^1.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4}

(Williams et al., 2015)

hoip¹ has muscle cell of lateral transverse muscle 3 | embryonic stage phenotype, suppressible | partially by Tm2^1.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4}

(Williams et al., 2015)

hoip¹ has embryonic somatic muscle cell phenotype, suppressible | partially by Scer\GAL4^{kirre-rP298-G4}/Hsap\SNU13^UAS.cJa

(Johnson et al., 2013)

NOT suppressed by

Statement

Reference

hoip¹ has muscle cell of lateral transverse muscle 1 | embryonic stage phenotype, non-suppressible by Tm2^3.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4}

(Williams et al., 2015)

hoip¹ has muscle cell of lateral transverse muscle 2 | embryonic stage phenotype, non-suppressible by Tm2^3.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4}

(Williams et al., 2015)

hoip¹ has muscle cell of lateral transverse muscle 3 | embryonic stage phenotype, non-suppressible by Tm2^3.UAS.EGFP/Scer\GAL4^{kirre-rP298-G4}

(Williams et al., 2015)

hoip¹ has embryonic somatic muscle cell phenotype, non-suppressible by Mhc^+memb

(Johnson et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Tm2^{1.Scer\UAS.T:Avic\GFP-EGFP} or Tm2^{2.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^kirre-rP298 when expressed at high levels is able to significantly partially rescue the lateral longitudinal and lateral transverse muscle myotube elongation defects of hoip¹ mutant embryos, and when expressed at low levels is able to partially rescue the lateral transverse (but not lateral longitudinal) muscle myotube elongation defects of hoip¹ mutant embryos.

Expression of Tm2^{3.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^kirre-rP298 when expressed at high levels is able to significantly partially rescue the lateral longitudinal (but not lateral transverse) muscle myotube elongation, dorsal oblique muscle 2 size, sarcomere assembly and myoblast fusion defects of hoip¹ mutant embryos, but when expressed at low levels is unable to rescue the lateral longitudinal or lateral transverse muscle myotube elongation or sarcomere assembly defects of hoip¹ mutant embryos.

Expression of 2 copies of Tm2^{cDNA.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^kirre-rP298 is able to significantly rescue the lateral longitudinal and lateral transverse muscle myotube elongation defects of hoip¹ mutant embryos.

(Williams et al., 2015)

Expression of Mhc^+memb is unable to suppress the somatic muscle morphology defects seen in hoip¹ mutant embryos.

(Johnson et al., 2013)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\NHP2L1^Scer\UAS.cJa in founder cells under the control of Scer\GAL4^kirre-rP298 significantly rescues the myotube elongation defects seen in hoip¹ mutant embryos.

(Johnson et al., 2013)

Complementation and Rescue Data

Fails to complement

hoip^k07104

(Johnson et al., 2013)

Rescued by

hoip¹ is rescued by hoip^UAS.cJa/Scer\GAL4^{kirre-rP298-G4}

(Johnson et al., 2013)

Comments

Expression of hoip^Scer\UAS.cJa under the control of Scer\GAL4^kirre-rP298 rescues the myotube elongation defects seen in hoip¹ mutant embryos.

(Johnson et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

hoip¹

(Williams et al., 2015, Johnson et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (2)

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